№ 4 (185) 2023 р. С. 75–82

БЕЗКЛІТИННІ БІОЛОГІЧНІ ЗАСОБИ: ФОКУС НА КОНДИЦІОНОВАНІ СЕРЕДОВИЩА МЕЗЕНХІМАЛЬНИХ СТОВБУРОВИХ КЛІТИН

Ф. В. Гладких

Харківський національний університет імені В. Н. Каразіна, Харків, Україна
Державна установа «Інститут медичної радіолоґії та онколоґії імені С. П. Григор’єва Національної академії медичних наук України», Харків, Україна
Інститут проблем кріобіолоґії і кріомедицини Національної академії наук України, Харків, Україна

DOI 10.32782/2226-2008-2023-4-15

Огляд присвячено узагальненню сучасних відомостей про кондиціоновані середовища мезенхімальних стовбурових клітин (КС-МСК), як інноваційний безклітинний біологічний засіб. Термін «кондиціоноване середовище» належить до рідкої фази серед- овища клітинної культури, збагаченої секретомом культивованих клітин. Характеристики КС-МСК різняться залежно від власне джерела вихідних МСК (кістковий мозок, жирова тканина, плацента та ін.), газових умов культивування – нормоксія (O2 20,0–21,0 %) або гіпоксія (0,5–1,0–1,5–2,0 % О2), тривалості культивування (від 16–24–48–72 годин до 3–5 днів) та ін. Культуральне середовище в культурі in vitro являє собою мікрооточення в умовах in vivo та може визначати долю клітин і, таким чином, їхні паракринні влас- тивості. Застосування КС-МС має переваги над власне МСК за рахунок відсутності імуногенності. Натепер у світі зареєстровано 14 клінічних досліджень щодо ефективності кондиціонованих середовищ.

Ключові слова: безклітинні біологічні засоби, кондиціоноване середовище, позаклітинні везикули, екзосоми, культиваційне середовище.

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