Abstract:
According to literature data, in the conditions of the balance of pro- and antiinflammatory factors, the development of the infectious-inflammatory process tends to progress and more severe with subsequent transformation into chronic inflammation with
corresponding morphofunctional effects. Under such critical conditions, an apoptosis is
likely to play a crucial protective role, which can eliminate the excessive accumulation
of aggressive inflammatory effects and effectively eliminate them, which in the future
prevents the probable transformation into a persistent form. The aim of the work was to
study the features of the early dynamic processes of apoptosis in the tissues of the
kidneys under simulation conditions in the experiment of acute pyelonephritis and
concomitant diabetes mellitus type I and II. The purpose of the study was to study the
features of the early dynamic processes of apoptosis in the tissues of the kidneys under
simulation conditions in the experiment of acute pyelonephritis and concomitant type I
and type II diabetes. The work was performed on 300 adult Wistar rats, which were
divided into 4 groups. Fragments of animal's kidneys were studied and photographed in
an electron microscope PEM-100-01. The results showed that after modeling in the
animals of pyelonephritis in the nephrons there were no significant ultrastructural changes.
The structure of the podocytes of the outer sheet was almost the same as the structure
of the podocytes of the control material, and in some cells there were signs of activation
of their metabolic activity. In the structure of the podocytes of the inner leaf of the capsule,
dystrophic changes of the internal membrane of the mitochondria were established. In
kidney medulla, the structure of the glomerulus was more preserved than in the cortical.
Pathological changes of the proximal and distal tubular podocytes, as well as interstitial
tissue, are more pronounced than the renal glomeruli. Under the modeling of the common
model of pyelonephritis and type 1 diabetes, more pronounced morphological changes
occur: destructive changes in the endothelial cells of the glomerular capillaries, the
homogenization of the structure of the basement membrane occurs and the mesangial
tissue is significantly enlarged. In nephrons and tubules of cortex, changes are manifested
to a much greater extent than in the kidney medulla. When studying in clinical conditions
the pathogenetic features of acute pyelonephritis in conditions of concomitant diabetes
mellitus it is expedient to carry out electron microscopic research with the aim of choosing
the optimal corrective therapeutic effect and preventing the unfavorable course of
infectious and inflammatory process and its transformation into persistent form. EM
(electron-microscopic) studies are highly informative in the study of pathological changes
and early dynamic processes of apoptosis in renal tissues in the design of acute
pyelonephritis and concomitant diabetes mellitus I and II in the experimental conditions.
