N 2 (199) 2026. P. 69–74

EVALUATION OF THE INFORMATIVENESS OF BIOMARKERS FOR PYELONEPHRITIS PROGRESSION IN PATIENTS WITH DIABETES MELLITUS

Odesa National Medical University, Odesa, Ukraine

State Institution “The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine”, Odesa, Ukraine

Goodweb IT company, Chornomorsk, Ukraine

DOI 10.32782/2226-2008-2026-2-10

The aim of the work is to conduct the analysis and study the correlation between the levels of methylglyoxal, a marker of early protein glycation-β-2-microglobulin – a protein that indicates the presence of damaged renal tubules, and the leukocyte intoxication index in the peripheral blood of somatically healthy people and patients with acute pyelonephritis and type II diabetes mellitus.

The results obtained indicate the presence of a pronounced positive relationship between the indicators of the inflammatory process marker in AP and concomitant diabetes.

Conclusions. A statistically significant increase in the level of MGL and β-2 – microglobulin in blood plasma was established in patients with AP and concomitant diabetes, which indicates the intensification of protein glycation processes in the body and damage to protein ultrastructures in kidney tissues and indicates the important role of concomitant diabetes in the development of functional renal complications in the pathological condition under study. A significant increase in the level of LII in the blood of patients with AP with concomitant diabetes was found, which indicates the pathogenetic role of concomitant diabetes in the development of an unfavorable course of the inflammatory process in the kidneys. The revealed significant correlation coefficient in AP and concomitant diabetes in blood between the levels of MGL and β-2-microglobulin and MGL and the LII index, as well as between the values of β-2-microglobulin and LII, indicates the possibility of using these indicators as biomarkers, in particular β-2-microglobulin and LII, to assess the nature and predict the course of the pathological condition under study.

Keywords: pyelonephritis, diabetes, β-2-microglobulin, intoxication index.

REFERENCES

1. Herness J, Buttolph A, Hammer NC. Acute Pyelonephritis in Adults: Rapid Evidence Review. Am Fam Physician. 2020;102(3):173–180. PMID: 32735433.
2. Nabi T, Rafiq N, Rahman MHU, Rasool S, Wani NUD. Comparative study of emphysematous pyelonephritis and pyelonephritis in type 2 diabetes: a single-centre experience. J Diabetes Metab Disord. 2020;19(2):1273–1282. https://doi.org/ 10.1007/s40200-020-00640-y.
3. Gutierrez-Mariscal FM, Podadera-Herreros A, Alcalá-Diaz JF, et al. Reduction of circulating methylglyoxal levels by a Mediterranean diet is associated with preserved kidney function in patients with type 2 diabetes and coronary heart disease: From the CORDIOPREV randomized controlled trial. Diabetes Metab. 2024;50(1):101503. https://doi.org/10.1016/j.diabet.2023.101503.
4. Lai SWT, Hernandez-Castillo C, Gonzalez EJL, et al. Methylglyoxal Adducts Are Prognostic Biomarkers for Diabetic Kidney Disease in Patients with Type 1 Diabetes. Diabetes. 2024;73(4):611–617. https://doi.org/10.2337/db23-0277.
5. Canlar-Akar D, Kiral F. Evaluation of renal damage biomarkers in an experimental Pyelonephritis model induced by uropathogenic Escherichia coli. Rev Cient FCV-LUZ. 2025;35(2):7. https://doi.org/10.52973/rcfcv-e35606.
6. Yamashita R, Izumi Y, Nakada K, et al. Utility of urinary presepsin in the diagnosis of pyelonephritis: a cross-sectional study.
7. Cheung CL, Lam KS, Cheung BM. Serum β-2 microglobulin predicts mortality in people with diabetes. Eur J Endocrinol. 2013;169(1):1–7. https://doi.org/10.1530/EJE-13-0003.
8. Zahoruyko RR. Leykotsytarnyy indeks intoksykatsiyi pry zapalnykh protsesakh verkhnikh sechovyvidnykh shlyakhiv [Leukocyte intoxication index in inflammatory processes of the upper urinary tract]. Zdorov’ya Cholovika. 2016;(3):156–159 (In Ukrainian). https://health-man.com.ua/2412-5547/article/view/104799/99893.
9. Argyropoulos CP, Chen SS, Ng YH, et al. Rediscovering Beta-2 Microglobulin as a Biomarker across the Spectrum of Kidney Diseases. Front Med (Lausanne). 2017;4:73. https://doi.org/10.3389/fmed.2017.00073.
10. Borysov SO, Kolosov OM, Borysov OV, Kostyev FI. Doslidzhennya vmistu beta-2-mikrohlobulinu v plazmi krovi ta sechi pry hostromu piyelonefryti ta suputnomu tsukrovomu diabeti v eksperymenti za umov medykamentoznoho vplyvu [Study of beta-2-microglobulin content in blood plasma and urine in acute pyelonephritis and concomitant diabetes mellitus in an experiment under the conditions of medical influence]. Urolohiya. 2020;24(1):20–25 (In Ukrainian). https://doi.org/10.26641/ 2307-5279.24.1.2020.199492.
11. Borisov SO, Kostyev FI, Borisov OV, Kolomiichuk SG, Martyniuk DV. Monitoring the course of acute pyelonephritis complicated by diabetes mellitus in the experiment. Zdorov’ya Cholovika. 2024;(4):5–11 (In Ukrainian). https://doi.org/10.30841/ 2786-7323.4.2024.322109.
12. Borysov SO, Kostyev FI, Yakymenko OO, et al. Monitoring of cytotoxin – methylglyoxal as a marker of the pathogenesis of acute pyelonephritis in diabetes mellitus. Odesa Med J. 2025;(1):9–13 (In Ukrainian). https://doi.org/ 10.32782/2226-2008-2025-1-1.
13. Burlaka IA, Mityuryayeva IO, Ipatii NS, Smochko MYu, Kovalchuk IV. Dynamics of clinical, biochemical and therapeutic indicators in the course of diabetic nephropathy in a child: case report. Clin Prev Med. 2024;7:83–88. https://doi.org/ 10.31612/2616-4868.7.2024.10.
14. Vikhrova IO, Loboda AM, Zmyslia IF. Significance of urinary Aminopeptidase N and Dipeptidyl peptidase iv in early diagnosis of kidney damage in children with type 1 diabetes mellitus in North-Eastern region of Ukraine. East Ukr Med J. 2024;12(4):808–817. https://doi.org/10.21272/eumj.2024;12(4):808-817.
15. Borysov SO, Kostyev FI, Borysov OV, et al. Monitorynh korelyatsiynoyi zalezhnosti diahnostychnykh markeriv hostroho piyelonefrytu ta tsukrovoho diabetu v umovakh eksperymentu [Monitoring the correlation of diagnostic markers of acute pyelonephritis and diabetes mellitus under experimental conditions]. Zdorov’ya Cholovika. 2025;(2):28-33. (In Ukrainian). https://doi.org/10.30841/2786-7323.2.2025.337327.
16. Wagenmakers E, Kucharský Š, van Doorn J, van den Bergh D. Accessible and Sustainable Statistics with JASP. OSF Preprints. 2023. https://doi.org/10.31234/osf.io/ud2vjю
17. Wang Y, Liu X, Xiao Z. Diagnostic accuracy of neutrophil-to-lymphocyte ratio in type 2 diabetic nephropathy: a meta-analysis. Front Endocrinol (Lausanne). 2025;16:1564170. https://doi.org/10.3389/fendo.2025.1564170.
18. Horovyi VI, Holovenko VP, Kobzyn OL. Osoblyvosti likuvannia khvorykh iz hniinym piielonefrytom, uskladnenym septychnym shokom [Features of the treatment of patients with purulent pyelonephritis complicated by septic shock]. Urolohiya. 2010;(14):84–85 (In Ukrainian).