N 6 (197) 2025. P. 32–39

DIAGNOSTIC SENSITIVITY AND SPECIFICITY OF PRO- AND ANTIOXIDANT SYSTEM MARKERS IN MEN WITH ERECTILE DYSFUNCTION FOLLOWING COMBAT-RELATED INJURIES

М. Z. Vorobets, Z. Ya. Fedorovych, R. V. Fafula, D. Z. Vorobets, Z. D. Vorobets, O. M. Chemerys

State Non-Profit Enterprise “Danylo Halytsky Lviv National Medical University”, Lviv, Ukraine

DOI 10.32782/2226-2008-2025-6-4

Introduction. To study the predictive ability of using the quantitative value of the level of a single indicator to detect oxidative stress, ROC analysis was performed with the calculation of the optimal cut-off threshold selected according to the criterion of maximum balance between sensitivity and specificity.

The aim of this research is to analyze the diagnostic sensitivity and specificity of pro- and antioxidant system indicators in men injured as a result of combat operations.

Materials and methods. 116 samples of peripheral blood lymphocytes and serum from men, which were divided into two groups by age, were analysed for the sensitivity and specificity of pro- and antioxidant system indicators.

Results. The results of ROC analysis for indicators of the pro- and antioxidant system of lymphocytes and peripheral blood serum demonstrated their predictive ability for detecting oxidative stress. Malondialdehyde of blood lymphocytes for the age group 20–39 years, reduced serum glutathione and total glutathione of blood lymphocytes for all examined men were characterized by excellent discriminatory ability. Malondialdehyde of blood lymphocytes for the age group 20–39 years showed itself as a highly sensitive marker with a slightly lower specificity value. Reduced glutathione of blood serum of men of all age groups is characterized as a highly specific marker with a slightly lower sensitivity value. Total glutathione of blood lymphocytes is simultaneously characterized by high sensitivity and specificity for all examined men.

Conclusions. The concentration of malondialdehyde in blood lymphocytes and the levels of total and reduced glutathione in blood serum were the most significant predictors for the detection of oxidative stress based on the ROC analysis. These markers demonstrate high sensitivity and specificity as indicators of the pro- and antioxidant balance.

Key words: erectile dysfunction, combat trauma, pro- and antioxidant system, ROC analysis.

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