N 5 (196) 2025. P. 96–101

THE STUDY OF FATTY ACID COMPOSITION IN RUMEX PATIENTIA L. × RUMEX TIANSCHANICUS LOSINSK

L. V. Slobodianiuk, S. M. Marchyshyn, O. M. Horoshko,
L. V. Kostyshyn, L. I. Budniak

Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine, Ternopil, Ukraine,
Bukovinian State Medical University, Chernivtsi, Ukraine

Introduction. Rumex patientia L. × Rumex tianschanicus Losinsk. is a multifunctional plant valued for its use as forage, food, vegetable, and medicinal resource. It provides a rich source of plant-derived nutrients, including high-quality protein, essential macro- and micronutrients, vitamins, organic acids, lipids, amino acids, and carotenoids. Therefore, an in-depth phytochemical study of all organs of this plant is relevant. The aim of our study was to conduct a comparative analysis of the content of fatty acids in the leaves, flowers, roots, and seeds of Rumex patientia L. × Rumex tianschanicus Losinsk.

Materials and methods. The material for the research was the leaves, roots, seeds and flowers of Rumex patientia L. × Rumex tianschanicus Losinsk. Identification and quantitative content of fatty acids in the studied medicinal plant raw materials was carried out by gas chromatography / mass spectrometry (GC/ MS) of fatty acid methyl esters using the Agilent 6890N/5973inert gas chromatography-mass spectrometry system (Agilent technologies, USA).

Results. Some 15 fatty acids have been identified in Rumex patientia L. × Rumex tianschanicus Losinsk. roots, leaves, flowers, and seeds, and their quantitative content has been determined by gas chromatography. Accordingly, in all samples of Rumex patientia L. × Rumex tianschanicus Losinsk., unsaturated fatty acids dominated. Oleic acid was the dominant unsaturated fatty acid in the flowers and seeds, while α-linolenic acid was predominant in the roots. The highest content of oleic acid (7947.51 µg/g) was found in the seeds, while that of linoleic acid (1507.01 µg/g) was found in the flowers.

Conclusion. The performed study on the fatty acid composition of Rumex patientia L. × Rumex tianschanicus Losinsk. did not reveal any substantial differences in qualitative composition and quantitative content of fatty acids across different plant parts. The results have shown that unsaturated fatty acids prevailed in the roots, leaves, flowers, and seeds.

Keywords: Rumex patientia L. × Rumex tianschanicus Losinsk., fatty acids, gas chromatography/mass spectrometry.

REFERENCES

  1. Budniak L, Slobodianiuk L, Marchyshyn S, Potishnyi I. Determination of amino acids of plants from Angelica genus by HPLC method. Pharmacia. 2022; 69(2): 437–446. DOI: 10.3897/pharmacia.69.e83705.
  2. Prystupa BV, Bogatu SI, Eberle LV, Rozhkovsky YaV, Vorobyov DA. Farmakolohichnyi ta farmakohnostychnyi analiz roslyn rodu stellaria [Pharmacological and pharmacognostic analysis of plants of the genus Stellaria]. Odesa Medical Journal. 2025; 2(193): 96–102. DOI: 10.32782/2226-2008-2025-2-15 (In Ukrainian).
  3. Moroz PA, Cherevchenko TM, Rakhmetov DB. Stanovlennia ta rozvytok selektsiinykh doslidzhen u Natsionalnomu botanichnomu sadu im. M. M. Hryshka NAN Ukrainy [Formation and development of selection research in the National Botanical Garden named after M.M. Grishko of the NAS of Ukraine]. Introduction of Plants. 2003; 4: 37–46. (In Ukrainian). Available from: http://jnas.nbuv.gov.ua/article/UJRN-0001037618.
  4. Li JJ, Li YX, Li N, Zhu H-T, Wang D, Zhang Y-J. The genus Rumex (Polygonaceae): An ethnobotanical, phytochemical and pharmacological review. Prod. Bioprospect. 2022; 12: 21. DOI: 10.1007/s13659-022-00346-z.
  5. Berillo D, Kozhahmetova M, Lebedeva L. Overview of the Biological Activity of Anthraquinons and Flavanoids of the Plant Rumex Species. Molecules. 2022; 27: 1204. DOI: 10.3390/molecules27041204.
  6. Litvinenko YA, Muzychkina RA. Phytochemical Investigation of Biologically Active Substances in Certain Kazakhstan Rumex Species. 1. Nat. Compd. 2003; 39: 446–449. DOI: 10.1023/B:CONC.0000011117.01356.4c.
  7. Feduraev P, Skrypnik L, Nebreeva S, et al. Variability of Phenolic Compound Accumulation and Antioxidant Activity in Wild Plants of Some Rumex Species (Polygonaceae). Antioxidants (Basel). 2022; 11(2): 311. DOI: 10.3390/antiox11020311.
  8. Zengyang He, Jianfeng Zou, Xinyao Li, Tao Jiang, Jianguo Zeng, Miaofen Chen. Responses of nitrogen metabolism, photosynthetic parameter and growth to nitrogen fertilization in Rumex patientia × Rumex tianschanicus Losinsk. Chilean journal of agricultural research. 2025; 85(3): 459–468. DOI: 10.4067/s0718-58392025000300459.
  9. Rolinec M, Rakhmetov D, Biro D, et al. Energy content of hybrid Rumex patientia x Rumex tianschanicus A.Los (Rumex OK 2) samples from spring months and June. Acta Fytotechnica et Zootechnica. 2018; 21(2): 60–62. DOI: 10.15414/ afz.2018.21.02.60-62.
  10. Rakhmetov DB, Rakhmetova SO. New ultra-early culture of complex use. Proposal. 2008; 3: 62–70. (In Ukrainian). Available from: https://propozitsiya.com/articles/nova-ultrarannya-kultura-kompleksnoho-vykorystannya.
  11. Feshchenko H, Oleshchuk O, Slobodianiuk L, Milian I. Study of Epilobium angustifolium amino acids content by HPLC method. ScienceRise: Pharmaceutical Science. 2021; 6(34): 85–90. DOI: 10.15587/2519-4852.2021.249836.
  12. Atolani O, Adeniyi O, Kayode OO, Adeosun CB. Direct preparation of fatty acid methyl esters and determination of in vitro antioxidant potential of lipid from fresh Sebal causarium Seed. Journal of Applied Pharmaceutical Science. 2015; 5: 24–28. DOI: 10.7324/JAPS.2015.50305.
  13. Slobodianiuk L, Budniak L, Feshchenko H, Sverstiuk A, Palaniza Y. Quantitative analysis of fatty acids and monosaccharides composition in Chamerion angustifolium by GC/MS method. Pharmacia. 2022; 1: 167–174. DOI: 10.3897/pharmacia.69. e76687.
  14. Olefirenko AO, Kyslychenko VS. Doslidzhennia zhyrnokyslotnoho skladu travy liziantusu rassela [Research of the fatty acid composition of russell’s lisianthus herb]. Odesa Medical Journal. 2024; 6(191): 92–95. (In Ukrainian). Available from: https://doi.org/10.32782/2226-2008-2024-6-16.
  15. Kim J, Kim Y, Kim Y, Park Y. Conjugated linoleic acid: potential health benefits as a functional food ingredient. Annual review of food science and technology. 2016: 7: 221–44. DOI: 10.1146/annurev-food-041715-033028.
  16. Mercola J, D’Adamo CR. Linoleic Acid: A Narrative Review of the Effects of Increased Intake in the Standard American Diet and Associations with Chronic Disease. Nutrients. 2023; 15(14): 3129. DOI: 10.3390/nu15143129.
  17. Tamkanat T, Nawab F, Usmani QI, Sarwath S. Khaksi (Sisymbrium irio ): An updated review of its ethnopharmacology, phytochemistry and pharmacology. Journal of Drug Delivery and Therapeutics. 2025; 15(6): 288–296. DOI: 10.22270/jddt. v15i6.7219.
  18. Wang Q, Zhang H, Jin Q, Wang X. Effects of Dietary Plant-Derived Low-Ratio Linoleic Acid/Alpha-Linolenic Acid on Blood Lipid Profiles: A Systematic Review and Meta-Analysis. Foods. 2023; 12(16): 3005. DOI: 10.3390/foods12163005.
  19. Labdelli A, Tahirine M, Foughalia A, et al. Effect of Ecotype and Environment on Oil Content, Fatty Acid, and Sterol Composition of Seed, Kernel, and Epicarp of the Atlas Pistachio. Agronomy. 2022; 12(12): 3200. DOI: 10.3390/agronomy12123200.
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