N 5 (196) 2025. P. 83–89

EFFECT OF TAGETES PATULA L. EXTRACT ON LIVER AND PANCREAS HISTOSTRUCTURE IN MODELS OF ETHANOL-PARACETAMOL HEPATITIS AND STREPTOZOTOCIN DIABETES IN RATS

N. A. Tsubanova, N. I. Voloshchuk, H. B. Halevych

Communal Institution of Higher Education of Lviv Regional Council “Andrei Krupynskyi Lviv Medical Academy”, Lviv, Ukraine,
National Pirogov Memorial Medical University, Vinnytsia, Ukraine

DOI 10.32782/2226-2008-2025-5-14

Drugs are increasingly becoming predictors of the development of liver and pancreatic lesions. Drug-induced lesions are usually characterized by strong oxidative stress and destruction of hepatocyte and pancreatic cell membranes. Modern therapeutic strategies are primarily aimed at reducing clinical symptoms and are not able to exert an organoprotective effect. Medicinal plant raw materials with antioxidant and cytoprotective effects can be considered a promising object for reducing the toxic effect of some drugs on the liver and pancreas.

The purpose of this work was to evaluate the effect of extract herb Tagetes patula L (EHTP) on changes in histological parameters under conditions of ethanol-paracetamol hepatitis and streptozotocin diabetes.

Materials and methods. Experimental ethanol-paracetamol hepatitis and streptozotocin diabetes were modeled in rats using standard methods. The investigated EHTP and the reference drug Silymarin were administered at a dose of 25 mg/kg in a therapeutic and prophylactic regimen. Organ samples (liver in the first experiment and pancreas in the second) were fixed in 10% formalin, dehydrated in ascending grades of alcohol, embedded in paraffin. Sections 6–7 μm thick were obtained using a sliding microtome, mounted on glass slides, stained with hematoxylin and eosin.

Results. The EHTP has a noticeable protective effect under the conditions of ethanol-paracetamol hepatitis, which is manifested in a significant reduction in the degree of hydropic and fatty degeneration of hepatocytes, disorders of internal hemodynamics, inflammatory reaction; normalization of the structure and morphological integrity of cells. In the model of streptozotocin diabetes, the EHTP reduces destructively dystrophically altered pancreatic cells and exhibits anti-inflammatory effects, significantly reducing signs of insulitis and inflammatory reaction in the interacinar tissue.

Conclusions. The EHTP has a significant organoprotective effect, with a protective effect on hepatocytes in conditions of ethanol-paracetamol hepatitis and on pancreatic cells in conditions of streptozotocin diabetes.

Keywords: Tagetes patula L herb extract, ethanol-paracetamol hepatitis, streptozotocin diabetes, rat, histological examination.

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