N 4 (189) 2024. P. 90–94

CHOICE OF THE OPTIMAL METHOD OF EXTRACTION OF FLAVONOIDS AND POLYPHENOLS OF CENTAURIUM ERYTHRAEA RAFN. HERB

L. I. Budniak, S. M. Marchyshyn, L. V. Slobodianiuk, R. B. Kotsyuba,
N. Ya. Muzyka, P. V. Kryvosh

Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine, Ternopil, Ukraine
Bukovinian State Medical University, Chernivtsi, Ukraine

DOI 10.32782/2226-2008-2024-4-15

Introduction. Using knowledge of traditional medicine and recent scientific advancements, a significant number of medicines based on biological active substances from medicinal plants have been developed. Important biological active substances with pronounced pharmacological effects are flavonoids and polyphenols. Therefore, the aim of the study was to investigate the effect of the method of extracting total flavonoids and total polyphenols from Centaurium erythraea Rafn. herb.

Materials and methods. The object of the study was the Centaurium erythraea Rafn. herb. 69% ethanol was used as the extractant, with a raw material to extractant ratio of 1:5. Extracts from common centaury herb were obtained using the following methods: maceration, remaceration, maceration with stirring, and ultrasonic extraction. The evaluation criterion was the yield of total polyphenols and total flavonoids. The HPLC method was used to determine the qualitative composition and quantify the individual flavonoids.

Results. The research results showed that the maceration with stirring method extracted the highest total amounts of polyphenols ((18.2±0.19) mg/ml) and flavonoids ((16.57±0.19) mg/ml) from the investigated raw material. The qualitative composition and quantitative content of individual flavonoids were determined in the extract obtained via this method. It was found that the predominant flavonoid was neohesperidin (641.44 µg/ml). Rutin (217.07 µg/ml), kaempferol (42.36 µg/ml), quercetin (24.58 µg/ml), quercetin 3-O-beta-D-glucoside (22.16 µg/ml), and kaempferol 3-O-beta-D-glucoside (10.83 µg/ml) were also detected in slightly smaller amounts.

Conclusions. The highest amount of total flavonoids and total polyphenols was extracted from extracts obtained by the maceration with stirring method. Among the individual flavonoids determined by the HPLC method, neohesperidin prevailed.

Key words: Centaurium erythraea Rafn., herb, flavonoids, polyphenols, HPLC.

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