Relevance. Now the etiological factors of the disease are already known, the mechanisms of formation of exogenous allergic alveolitis are not fully understood. In particular, the questions concerning the functional state and role of prooxidant and antioxidant systems in the pathogenesis of experimental allergic alveolitis, especially in different periods of the development of the disease, remain unexplored. In the available literature, there are no studies of LPO parameters and antioxidant system activity in the thymus in experimental allergic alveolitis and Korvitin correction. The problem of exogenous allergic alveolitis is urgent and requires further experimental and clinical studies.
Purpose of the study. To estimate the state of the antioxidant system and the processes of LPO in the thymus of guinea pigs with experimental allergic alveolitis under conditions of immobilization stress in different periods of its formation before and after the use of the Korvitin.
Material and methods of investigation. There were three groups of guinea pigs (n=12): 1st — control, 2nd — EAA under gauze stress conditions on the 34th day of the experiment; 3rd — EAA under conditions of gauze stress on the 34th day of the experiment, which within 10 days was administered intraperitoneally with the drug Korvitin (Borshchagovsky Chemical-Pharmaceutical Plant) at a dose of 40 mg/kg. Then the animals were decapitated and the level of LPO and activity of antioxidant system (AOS) enzymes were determined in the thymus.
Results of the study. The MDA level increased by 80.44% compared to the control (p<0.05) at the 34-day test. DK level was not reliable in comparison with the first group of animals. Also, a significant decrease in the activity of antioxidant enzymes in guinea pigs was observed in conditions of combined pathological processes of EAA and immobilization stress. The activity of SOD decreased relative to the control by 55.8% (p<0.05). So in the thymus, the activity of SOD increased significantly by 36.3% (p<0.05), CT activity by 40.4% (p<0.05). The level of MDA after the administration of Korvitin significantly decreased in the thymus of guinea pigs. Thus, the MDA content decreased by 43.48% relative to the animals of the second group (p<0.05), and the DK level was not reliable.
Conclusions. At late stages of development of EAA in conditions of immobilization stress, corvitin showed a pronounced antioxidant effect, inhibited free-radical processes. All this makes a reasonable and adequate correction of Korvitin changes in the processes of LPO and AOS in the thymus of animals in later periods of EAA development and stress. Thus, polytropnism of pharmacodynamic effects with simultaneous absence of side effects allows us to state that Korvitin is one of the most prospective for the study of modern drugs of pharmacotherapy of many pathological processes, in particular EAA in conditions of immobilization stress.