Introduction. Clinicians and experimentalists pay much attention to role and importance of free radical oxidation (FRO) processes and antioxidant system (AOS) condition in the pathogenesis of experimental allergic alveolitis (EAA) development combined with adrenal myocardial damage (AMD).
As for today, tiotriazoline impact on the level of lipid peroxidation products (LPO) and AOS in the lungs during experimental allergic alveolitis and adrenal myocardial damage is not defined.
Purpose of our study was to ascertain the damage of prooxidative and antioxidant system conditions in the lungs during EAA and install AMD and ascertain the influence of tiotriazoline.
Materials and methods. Experiments were carried out upon 68 guinea pigs, which were divided into six groups. The first group is the control one; the second, third, fourth and fifth groups — animals with EAA and AMD on the first, seventh, fourteenth and twenty-fourth day of the experiment relevantly. The sixth group is guinea pigs with EAA and AMD after tiotriazoline intramuscular injections once a day at a dose of 100 mg per 1 kg and during 10 days (from 14th to 24th day).
Animals with EAA and AMD were decapitated at 1st, 7th, 14th and 24th day before and after tiotriazoline treatment and their lung tissue was taken for biochemical studies.
Results and discussion. Experimental studies showed that while the development of allergic alveolitis during AMD there is a gradual increase of diene conjugates and malondialdehyde level in the lungs.
Excessive formation of lipid peroxidation products caused a change in the activity of certain enzymes in lungs antioxidant system. It was found out that on the 1st day of the experiment superoxide dismutase and catalase in the lungs increase, and later, on the 7th, 14th and 24th day of EAA and AMD development, these enzymes activity decreases.
The obtained data allow us to witness the primary compensatory function of antioxidant protection with its subsequent depletion.
Application of tiotriazoline during 10 days resulted in a decrease in diene conjugates and malondialdehyde content and increased activity of superoxide dismutase and catalase, which signifies the corrective action to these parameters.
Conclusions. The obtained results allow to suggest that antioxidant tiotriazolin has the corective impact to the formation of lipid peroxidation products (diene conjugates and malondialdehyde) and antioxidant system activity (superoxide dismutase and catalase) during the development of AA and AMD.