The impact of hormone-vitamin complex on functional activity of the muscle tissue of descendants of irradiated animals

Abstract

The aim: To determine the hormone-vitamin complex impact on the terminal links of glycolysis, the tricarboxylic acids cycle, and the initial stage of gluconeogenesis in the muscle tissue in descendants of irradiated animals. Materials and methods: Pyruvate kinase, lactate dehydrogenase, malate dehydrogenase, NADP-dependent malate dehydrogenasee and phosphoenolpyruvate carboxykinase activities, the content of lactate, pyruvate, malate and oxaloacetate were determined in the blood, myocardium and thigh muscles of 66 rats after exposure to ionizing gamma-radiation. Rats were injected by a hormone-vitamin complex which efficacy was determined using the abovementioned indexes. Results: Hormone-vitamin complex administration to descendants of irradiated animals exposed to 1.0 Gy results to pyruvate kinase activity increase in the myocardium and skeletal muscles of descendants from animals irradiated by 0.5 Gy and exposed to 1.0 Gy irradiation. Blood serum pyruvate kinase activity in descendants from animals irradiated by 1.0 Gy and exposed to 1.0 Gy radiation after the pharmacological correction was higher compared with the same index before pharmacological correction. The lactate dehydrogenase activity in the myocardium, skeletal muscles and blood in descendants born from animals irradiated by maximal dose exposed to 1.0 Gy radiation was less in these tissues after pharmacological correction. Conclusions: The hormone-vitamin complex use in the descendants of irradiated animals led to muscle tissue energy resources improvement. Our data are the experimental background for theoriginal hormone-vitamin complex efficacy further evaluation in the aspect of vital organs and body systems functional activity restoration under the influence of ionizing radiation.