Therapeutic and preventive effects of polyfunctional antidisbiotics drugs on the condition of periodontal bone tissue of rats, who have consumed thermoperoxide sunflower oil for a long time

Abstract

Aim: to determine the effectiveness of the therapeutic and prophylactic action of various polyfunctional antidisbiotic drags (PFAD) on the state of the periodontal bone tissue of rats treated with thermoperoxide sunflower oil (TPSO). Methods: used sunflower oil, oxidized in the presence of copper ions at a temperature of +115 ºС for 2 hours. The following PFAD were used: kvertulin, lekvin, lekasil, lysozyme -forte. Rats were injected with food TPSO at a dose of 1 ml per rat for 75 days. PFAD was administered with food at a dose of 300 mg / kg, starting from day 31. The degree of dysbiosis in the tissues of the oral cavity, in the mucous membrane of the colon and in the liver was determined. In the bone tissue of the periodontium, the content of calcium, the activity of alkaline and acid phosphatases, as well, as the degree of atrophy of the bone tissue of the periodontium were determined. The mineralizing index MI was calculated from the ratio of phosphatase activities. Results: in rats treated with TPSO, the degree of dysbiosis significantly increased in all studied tissues. In bone tissue, the activity of alkaline phosphatase significantly decreases and the activity of acid phosphatase increases. The MI index sharply (2 times) decreases and the degree of atrophy increases significantly. The introduction of PFAD slightly increases the calcium content, significantly increases the alkaline activity and decreases the acid phosphatase activity. Of all PFAD, only lysozyme-forte significantly increases the MI index and reduces the degree of atrophy. Conclusion: long-term consumption of peroxide oil calls for the development of dystrophic processes in the bone tissue of the periodontium, which can be prevented with the help of PFAD, of which lysozyme-forte turned out to be the most effective.