The immobilization of lytic enzymatic complex from Streptomyces recifensis var. lyticus (sterilase) on the gauze with the help of hydrophilic polymers and polyvinyl alcohol, cross-linked by borax, jointly with alkaline protease, by entrapment in polymeric films is achieved. Preparations with a high retaining of lytic activity, stable at storage and g-irradiation, were obtained. The prospects of immobilized on the gauze sterilase usage as potential bacteriolytic preparation of LOR-organs lesions: chronic otites (mesotympanites, epitympanites), otites of fungal etiology and in ophthalmology in burn therapy, was shown.