N 1–2 (179–180) 2022. P. 54–58

THE PECULARITIES OF TUBERCULOSIS, CAUSED BY BEIJING STRAINS

Odessa National Medical University, Odesa, Ukraine

DOI 10.54229/2226-2008-2022-1-2-10

East Asian family Beijing M. tuberculosis is a family characterized by active distribution. The article analyzes the association between the features of the course of newly diagnosed tuberculosis in patients infected with pathogens of the Beijing family in comparison with the non-Beijing group. Cultures of M.tuberculosis for molecular genetic research were obtained from 87 patients with first diagnosed pulmonary tuberculosis. According to the presence of the IS6110 insertion fragment in the dnaA-dnaN region, 26,4% of the isolated cultures were reffered to the Beijing family. The medical records of 87 patients with pulmonary tuberculosis were analyzed. Patients infected with pathogens of the Beijing family more often has disseminated form of tuberculosis then infiltrative (60,9% to 30,5%, RR 2,0 CI 1,0 – 4,03), longer period of positive bacterioscopic and cultural tests for M.tuberculosis (RR 1, 8 CI 1,04 – 3,01), more common treatment failure. Higher frequency of mutations of the katG, inhA, rpoB genes, which cause resistance to isoniazid and rifampicin, and multiresistance was detected more often compared to non-Beijing pathogens. In the group of Beijing strains, the level of multiresistance detected by cultural method 31,6% versus 8.5% in non-Beijing, i.e. strains of the Beijing family were characterized by multiresistance 3.7 times more often according to the cultural study than isolates of the non-Beijing group (р<0.05; χ²=8.22) The obtained results permit to speculate that M. tuberculosis infection of the Beijing family is a risk factor for more severe corse of the disease.

Key words: tuberculosis, effectiveness of treatment, Beijing family, drug resistance, M.tuberculosis.

REFERENCES

1. Mycobacterium tuberculosis Beijing family: Analysis of the epidemiological and clinical factors associated with an emerging lineage in the urban area of Milan / F. Zanini et al. Infect Genet Evol. 2014; 25: 14–19.
2. Recent Transmission and Prevalent Characterization of the Beijing Family Mycobacterium tuberculosis in Jiangxi / China Dong Luo et al. Polish Journal of Microbiology. 2022; 71(3): 371–380.
3. Hanifeh Erie, Hami Kaboosi, Naeme Javid, Hesamaddin Shirzad-Aski, Masoumeh Taziki, Maya Babaee Kuchaksaraee, Ezzat Allah Ghaemi. The high prevalence of Mycobacterium tuberculosis Beijing strain at an early age and extra-pulmonary tuberculosis cases. Iranian Journal of Microbiology. 2017; 9(6): 312–317.
4. Chongguang Yang, Sobkowiak Benjamin, Naidu Vijay, Codreanu Alexandru. Phylogeography and transmission of M.tuberculosis in Moldova: A prospective genomic analysis. PLoS Medicine. 2022; 19(2): e1003933.
5. Dormans J., Burger M., Aguilar D. et al. Correlation of virulence, lung pathology, bacterial load and delayed type of hypersensitivity responses after infection with different Mycobacterium tuberculosis genotypes in a BALB/c mouse model. Clin.exp.immunol. 2004; 137(3): 460–468.
6. Tong Jingfeng, Meng Lu, Bei Cheng, Liu Qingyun, Wang Min. Modern Beijing sublineage of Mycobacterium tuberculosis shift macrophage into a hyperinflammatory status. Emerging Microbes and Infections. 2022; 11(1): 715–724.
7. Boshoff H.I., Barry C.E. Tuberculosis – metabolism and respiration in the absence of growth. Nat. Rev.Microbiol. 2005; 3: 70–80.
8. Reed M.B., Gagneux S., Deriemer K. et al. The W-Beijing lineage of Mycobacterium tuberculosis overproduces triglycerids and has the DosR dormancy regulon constitutively upregulated. J.Bacteriol. 2007; 189(7): 2583–258.
9. Tsapenko Y.P., Boyko M.G., Alieva N.M., Krasnoshapka Y.O., Buslyk T.V., Krayevska O.O., Tykhomyrova A.I. Course of lungs tuberculosis in case of infecting with М.tuberculosis strains of Beijing genus among firstly diagnosed patients of Poltava region. World of Medicine and Biology. 2013; 2: 187–189.
10. Konstantynovska Olha, Poteiko Petro, Rogozhin Anton, Liashenko Oleksandr, Solodiankin Oleksii, Sapko Svitlana. Features of different M.tuberculosis strains that were isolated from MDR-TB patients in Kharkiv, Ukraine. European Respiratory Journal. 2016; 48.
11. Banu S., Stephen Gordon V., Palmer Si et al. Genotypic Analysis of Mycobacterium tuberculosis in Bangladesh and Prevalence of the Beijing Strain. J. Clin. Microbiol. 2004;42(2):674–682.
12. Aftab Ayma, Afzal Samia, Qamar Zahida, Idrees Muhammad. Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan. J Clin Microbiol. 2002; 2: 2509–2512.
13. Herrera-Leon Laura, Molina Tamara, Saiz Pilar, Saez-Nieto Juan Antonio, Jimenez Maria Soledad. New multiplex PCR for rapid detection of isoniazid-resistant Mycobacterium tuberculosis clinical isolates. Antimicrobial agents and chemotherapy. 2005; 49(1): 144–147.
14. Antonenko P.B., Kresyun V.I., Filuk V.V., Antonenko K.O., Stokich V.G., Korotich N.Ya. Current status of drug-resistance of M.tuberculosis and posibility of its genotyping detection. World of Medicine and Biology. 2014; 3(45): 8–13.
15. Antonenko P.B., Kresyun V.I., Antonenko K.O., Antonenko .PB. Alternative genotyping of drug-resistant Mycobacterium tuberculosis strains. Polish Journal of Microbiology. 2014; 63(2): 249–253.
16. Merker Matthias, Nikolaevskaya Elena, Kohl Thomas A. et al. Multidrug- and Extensively Drug-Resistant Mycobacterium tuberculosis Beijing Clades, Ukraine. 2015. Emerg Infect Dis. 2020; 26(3): 481–490.
17. Parwati I., Alisjahbana B., Apriani L. et al. Mycobacterium tuberculosis Beijing Genotype Is an Independent Risk Factor for Tuberculosis Treatment Failure in Indonesia. J. Inf. Dis. 2010; 201: 553–557.
18. Parwati I.R. van Crevel, D. van Soolingen. Possible underlying mechanisms for successful emergence of the Mycobacterium tuberculosis Beijing genotype strains Lancet Infect Dis. 2010; 10(2): 103–111.
19. Liu Q., Wang D., Martinez R., Lu P., Zhu L., Lu W., Wang J. Mycobacterium tuberculosis Beijing genotype strains and unfavourable treatment outcomes: a a systematic review and meta-analysis. Clinical Microbiology and infection.2020; 26(2): 180–188.