It was studied the antagonistic influence of the Lactobacilli (Lactobacillus murinus and Lactobacillus rhamnosus) on the Candida fungi and possibility of antimycotics usage in composition with Lactobacilli, on the basis of which probiotics are developed. It was shown that Lactobacilli have fungicidal activity towards C. albicans, C. tropicalis and C. krusei. The synergism of antimycotic activity of selected Lactobacilli with antimycotic agents was confirmed.