By phylogenetic analysis of nucleotide sequences the order of duplication of homologues of human alcohol dehydrogenase genes ADH1A, ADH1B, ADH1C, ADH4, ADH5, ADH6 and ADH7 was determined by Maximum Likelihood method. A dendrogram was constructed according to result of conducted phylogenetic analysis. Evaluation of the evolutionary divergence between sequences of the studied genes was made.