Results of subacute toxification of white rats by phosphorus-containing detergents found the significant violations of the functional activity of sperm in a group of animals under the influence of 1/10 LD50. They reduced sperm motility. Analysis of the results showed that the decreasing the sperm number and increasing the dead form gametes can be a significant contribution to violation of the generative function of rats under the influence of 1/100 LD50 and 1/10 LD50. Inhibition of the functional activity of sperm was confirmed also by osmotic inhibition of acid resistance and sperm. Analysis of subtoxic effect on morphological parameters gonad showed the inhibition index of spermatogenesis, the total spermatogonia, relative number of tubules from the meiosis 12-th stage. Along with this there was increasing number of desquamated tubular epithelium.