The goal of the present study was to evaluate the spreadness of rifampicin-resistance, spreadness of the mutations in 516/526/531 codons rpoB gene with the help of a multiplex allele-specific polymerase-chain reaction (PCR) assay, and develop screening method of rifampicin-resistance detection.
The described method of detection of rpoB mutations can be recommended as screening method for rifampicin-resistance detection with the help of a multiplex allele-specific PCR assay.