The course of the diaphragm tissue regeneration processes has been studied by means of an experimental research carried out on 70 animals with a recreated model of hernias of the esophageal opening (HEO) under conditions of using of an elaborated method of HEO repair with a prolene implant (Patent of Ukraine N 17368). A pathohistological study of the biopsy materials obtained at different stages of the postoperative period proved the efficacy of the proposed technique.