Aim: Quantitative and qualitative examination of normal and conditionally-pathogenic microflora in vaginal secretion of 53 women without signs of infectious-inflammatory processes.
Materials, methods: Real-time PCR was used for examination of normal and conditionally-pathogenic microflora in vaginal contents.
Results and discussion: Among facultative anaerobes the microorganisms of Mobiluncus of spp. /Corynebacterium spp., Eubacterium spp. and Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp. prevailed in quantity. However, their amount wasn’t more than 2.2 lg of GE/ample. Amount of Atopobium vaginalis (marker of bacterial vaginosis) wasn’t more than 0.7 lg of GE/sample. Amount of Ureaplasma urealyticum + parvum wasn’t more than 1.6 lg of GE/sample. Representatives of Candida spp. encountered in 85% cases, but not higher than 3.2 lg of GE/sample. Representatives of Sneathia spp., Leptotrihia spp., Fusobacterium spp., and also Mycoplasma hominis + genitalium families weren’t revealed in normocenosis. While estimating microorganisms species total amount there was shown that facultative anaerobes prevailed and they comprised 35%, obligate anaerobes — 30%, Candida spp. — 25% and mycoplasma — 10%.
Conclusions. It was shown that in women with normocenosis the age and obstetric-gynaecological anamnesis did not influence statistically meaningful on qualitative and quantitative characteristics of vaginal microbiota species composition. There were offered limiting indexes of normal microflora’s different components.