It is known that diabetic nephropathy development rate ranges from 40 to 50% in patients with diabetes type 1 and from 15 to 30% — with diabetes type 2, and this complication is the main reason for death in patients with diabetes mellitus type 1, and 8% of patients with diabetes type 2 die from uremia. It was also established that the risk of cardiovascular events as the leading causes of mortality in patients with diabetes increases in kidney damage.
The aim of this investigation — to establish the influence of water-soluble form of quercetin — Corvitin — on the state of the kidneys under conditions of experimental diabetes with obesity.
Materials and methods. White male rats were used during the experiments. Experimental animals were divided into three groups: 1st — control (intact animals); 2nd — animals with diabetes; animals of 3rd group were injected with Corvitin (Borshchahivskyy chemical-pharmaceutical plant, Kyiv, in a dose 10 mg/kg, intraperitoneal daily, starting through 10 weeks after STZ administration, during 2 weeks). Simulation of diabetes mellitus was carried out by a single intraperitoneal administration of streptozotocin (STZ, Sigma, USA, 30 mg/kg of body weight) after the previous 4-week accepted in high fat diet. STZ was dissolved immediately before the introduction of a 0.1 molar citrate buffer (pH 4.5); animals from control group were injected with the appropriate amount of citrate buffer. Renal tissue was investigated. We determined: indicators of lipid peroxidation — lipid hydroperoxides, TBA-active products, diene conjugates, antioxidant status: superoxide dismutase, catalase, glutathione, NO2–, the enzyme activity of mitochondria — succinate dehydrogenase, cytochrome oxidase.