Introduction. Diabetic retinopathy pathogenesis includes mechanisms of the neoangiogenesis, which are dependent upon free oxygen radical generation and cytokines local effects. It is reasonabe to measure level of cytokines as well as markers of oxidative processes in blood of patients suffered from diabetes retinopathy under condition of treatment with antioxidants such as delta-sleep inducing peptide and not-invasive activation of cerebellar structures.
The aim of the investigation was to measure the level of cytokines, namely — vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), interleukin-8 (IL-8), interferon-gamma (IFN-gamma)-inducible protein-10 (IP-10), as well as thiobarbituric acid reactive substances (TBARS) and ascorbic acid in the serum of patients with proliferative diabetic retinopathy (PDR), who have been treated with deltalicin and transcranial cerebellar stimulations.
Methods of investigations. 15 healthy persons along with 72 insuline-dependent patients who suffered from 2-type of diabetes with PDR have been observed in the investigation. Deltalicin (0.0003 g of delta-sleep inducing peptide per ampoule) was administered intranasally daily during two months. Transcranial stimulations were delivered using “Neurо МS/D” device with the induction of magnetic field at the height of impulse of 2.0 Тl and friquency of biphasic impulses generation of 5 Hz. The duration os stimulation was 10 s.
Results. The net increasing of the content of VEGF — 1.92 times, PEDF — 1.78 times, IL-8 — 3 times, IP-10 — 2.43 times, as well as two-fold increasing of TBARS content and the reduction of ascorbic acid content by 3.8 times pertained to the corresponded indices in practically healthy persons have been registered in the serum of patients suffered from PDR. The treatment with deltalicin was resulted in the reduction of VEGF — by 27.3% (p<0.05), PEDF — by 25.2% (p>0.05), IL-8 — by 21.7% (p<0.05), IP-10 — by 46.2% (p<0.05) when compared with the corresponded data before treatment in patients with PDR. Besides, the level of TBARS was reduced by 26.1% (p>0.05), аnd the increasing of ascorbic acid level by 2.7 times (p<0.05) have been also observed. All investigated indices were significantly different from those ones which have been registered in healthy persons. The usage of transcranial stimulations with magnetic field impulses was followed by tendencies to positive dynamic of all investigated indices, which have not achieved the level of significant differences when compared with those ones before treatment. Combined usage of deltalicin and cerebellar transcranial stimulations was resulted in the pronounced positive dynamics and were different from initial data significantly (p<0.05). The content of IL-8 was only apparent exclusion. The content of VEGF was reduced by 35.0% (p<0.05) and TBARS content — 1.7 times pertained to initial level (p<0.05), and both indices were not significantly different from corresponded data in practically healthy persons (p>0.05).
Conclusions. The increasing of VEGF, PEDF, IL-8, IP-10, along with the content of TBARS and decreasing of ascorbic acid content in serum may be used as a markers of the proliferative diabetic retinopathy development. The treatment with the delta-sleep inducing peptide contained drugs resulted in positive therapeutic dynamics of the investigated indices, and this effect is enhanced by transcranial cerebellar stimulations with magnetic impulses.