DISTRIBUTION OF MUTATION SDF-1 3’A OF GENE CXCR-4 AMONG PEOPLE OF THE WESTERN REGION OFUKRAINE

Aims. A speed of AIDS epidemic process development depends on correlation between susceptible and resistant individuals in every single population. Recent studies have revealed a close connection between chemokine receptors and human immunodeficiency virus infection. Immune and genetic changes, that influentce on the level of cytokines, could affect the susceptibility to immunodeficiency virus infection or speed of progress of disorder after infection. It was determined a link between spread of immunodeficiency virus infection and SDF-1 3’A mutation of chemokine receptor gene of human.

The purpose of study is to determine the frequency of chemokine receptor gene mutation SDF-1 3’A, which leads to higher resistance to HIV-1 among people of the Western region ofUkraine.

Methods. It was used the samples of peripheral blood as a materials for research of 200 healthy people (50% men and 50% women). Extracted DNA was amplified by polymerase chain reaction (PCR). The PCR products were subsequently digested with the restriction enzyme Msp I for identification of SDF-1 3’A mutation.

Results. Obtained results about frequency of mutation SDF-1 3’A of CXCR-4 is 18.25%. It is higher than in other ethnic group among people from Western region of Ukraine. Heterozygotes mutation SDF-1 3’A in studied group was found in 30.5% of people, homozygotes — in 3% of all. Mutation SDF-1 3’A was found at the same amount both in females and males. Among studied group the CCR5del32 was combined with SDF-1 3’A (8 person), CCR2-64I with SDF-1 3’A (9 person) and combination of homozygotes mutation CCR2-64I and heterozygotes mutation SDF-1/3’A (1 person).

Conclusions. Thus, the obtained results and the frequency of mutations in chemokine receptors gene indicate the high genetic resistance to HIV-1 infection amongWestern Ukraine region people compared with other ethnic groups.