Actuality. Alimentary deprivation as a social phenomenon and as a factor that aggravates the development of some pathological processes has long attracted the attention of researchers. The inevitable changes in the functioning of organs and tissues during the starvation cause cell metabolism restruction and transition to endogenous nutrition. The complete alimentary starvation within a few days contributes to the inhibition of protein biosynthesis mechanisms and violations of various levels of protein metabolism regulation. Under conditions of increased catabolism normal metabolic cycles (urea and ornithine) are violated, leading to hyperammonemia. Justification for the use of the phytopreparation on the basis of medicago sativa sowing grass “Medicabol” with protein metabolism violations on the model of alimentary deprivation of rats is represented.
Materials and methods. Alimentary deprivation is an experimental model in preclinical studies of drugs and it is recommended to evaluate the possible pharmacological effects on the indicators of protein metabolism. The animals were investigated in order to reveal metabolic disorders that developed in the body on the background of a pathology model. The body weight and the weight of the internal organs, the contents of total protein in the tissues of the internal organs were evaluated, the contents of urea and creatinine were determined in blood serum and urine.
Results. The results obtained on the background of alimentary deprivation show that “Medycabol” has the properties of protein metabolism corrector under conditions of increased catabolism. This effect is associated with a multi-component composition of the phytopreparation. Firstly, with the presence of essential amino acids that are alimentary substrates, and in particular the amino acid citrulline, which plays an important role in the detoxification of ammonia which is formed during the process of decay of the nitrogen-containing compounds within the ornithine cycle. By activating this cycle the stimulation of ammonia decay and reduction of hyperammonemia develop. Secondly, the impact of “Medycabol” on the concentration of urea in blood is more likely connected with the presence in its composition of saponins, which, along with the reduction of urea excretion in the urine, can significantly increase the protein content in the blood plasma. Thirdly, correction of protein metabolism violations is also possible due to the medicago sativa flavonoids that provide the direct and indirect metabolic effects, especially on protein metabolism.
It was experimentally found that “Medicabol” in a dose of 25 mg/kg at complete alimentary starvation is able to influence the plastic and proteolytic processes in the body, providing a compensatory adaptive action. Application of “Medicabol” in the health care regime stipulates saving of body weight of rats, increases the protein content in the internal organs and skeletal muscles, prevents the development of negative nitrogen balance, significantly reducing the level of urea and creatinine in blood and in urine in comparison with the animals of the control diseases.