The aim of the study was to analyze the levels of allergen-specific IgE in allergic patients in Poltavaregion in 2012, considering the data of aero-pollen monitoring of our region and the study of TLR2 and TLR4 polymorphisms in these patients, talking into account the allergen-specific IgE range.
Allergen-specific IgE to 19 most widespread allergens (Soyabean, Milk, Eggwhite, Crab-fish, Shrimp mix, Peach, House dust, D. pteronyssinus, D. farinae, Cockroach mix, Dog epithelia, Cat epithelia, fungus Alternaria tenius, Alder and Birch pollen, White Oak pollen, Ragweed pollen, Mugwort pollen, Timothy grass pollen, Rye pollen) were determined by using the Polycheck test system (Germany). The study of SNPs 2258G/A and TLR2 gene 896A/G, 1196C/T gene TLR4 in patients with allergic diseases and high allergen-specific IgE was performed by means of PCR using specific oligonucleotide primers.
In view of the results of aero-pollen research inPoltavaand the performed monitoring of allergen-specific IgE, the major groups of causative allergens in the development of allergic diseases inPoltavapopulation were determined. They were the allergens of house dust, dermathofagoidic mites, dust-producing trees (alder and birch), as well as aeroallergen plants (especially ragweed, timothy and mugwort).
The obtained results indicate the association of TLR2 gene 2258G/A and TLR4 gene 896A/G polymorphisms with increased production of specific IgE in patients with allergic diseases which makes it possible to considere these single nucleotide replacements as the additional prognostic characteristic of individual susceptibility to these diseases.