N 6 (197) 2025. P. 9–16

LIPID PEROXIDATION AND OXIDATIVE PROTEIN MODIFICATION IN EXPERIMENTAL PERIODONTITIS OF BACTERIAL-IMMUNE ORIGIN UNDER CONDITIONS OF METAL CROWN PLACEMENT (SERUM STUDY)

O. V. Marfiian, A. Ye. Demkovych

Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine, Ternopil, Ukraine

DOI 10.32782/2226-2008-2025-6-1

Studying changes in oxidative stress accompanying the placement of fixed structures in periodontitis will allow for a deeper understanding of the mechanisms of the pathological process and its prevention.

The aim of the work was to investigate disturbances in oxidative metabolism and the accumulation of peroxidation products in experimental periodontitis of bacterial-immune origin and under conditions of metal crown fixation.

Materials and methods. Experimental periodontitis of bacterial-immune origin was induced in animals by injecting a suspension of microorganisms mixed with egg protein into the periodontal tissues. On the 30th day of inflammation, the levels of diene and triene conjugates, products of oxidative protein modification, thiobarbituric acid-reactive compounds, and nitric oxide metabolites in blood serum were assessed using biochemical methods, while considering the fixation of both stamped and cast crowns.

Results and discussion. The results of the study indicate that after fixation of metal crowns, the level of lipid peroxidation decreases compared to the group without prosthetics. However, cast crowns demonstrate a greater effect in reducing oxidative stress than stamped ones, which may be due to their better adaptation to periodontal tissues and a lower reaction of soft tissues to this alloy. Moreover, even with the application of cast crowns, the level of oxidative stress remains elevated compared to control animals, highlighting the necessity for further studies aimed at optimizing the effects of metal prosthetic structures on periodontal tissue condition.

Conclusion. The development of the inflammatory process in periodontitis is accompanied by an increase in the level of lipid peroxidation in the blood serum, which is a marker of oxidative stress. After fixation of metal crowns, the level of oxidative stress is partially reduced, and cast crowns have a greater effect in reducing the intensity of lipoperoxidation than stamped ones.

Key words: prosthetics, stamped crowns, cast crowns, periodontitis, lipid peroxidation.

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