N 4 (195) 2025. P. 15–20

ADAPTIVE ACTION OF HEART CRYOEXTRACT ON MYOCARDIAL ENERGY METABOLISM IN EXPERIMENTAL EPINEPHRINE-INDUCED MYOCARDIODYSTROPHY

R. R. Komorovsky, M. O. Chyzh, F. V. Hladkykh, T. I. Liadova, M. S. Matvieienko

Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine, Ternopil, Ukraine
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
State Organization ”Grigoriev Institute for Medical Radiology and Oncology of the National Academy of Medical Sciences of Ukraine”, Kharkiv, Ukraine
N. Karazin Kharkiv National University, Kharkiv, Ukraine

DOI 10.32782/2226-2008-2025-4-2

Background. Myocardial energy homeostasis disruption plays a critical role in the development and progression of cardiovascular diseases. Epinephrine-induced myocardial dystrophy (EMD) serves as a relevant experimental model for studying the effects of sympathetic overactivation on myocardial tissue, including mitochondrial dysfunction, oxidative stress, and impaired carbohydrate metabolism.

Objective. This study aimed to elucidate the adaptive effects of porcine heart cryoextract on myocardial energy metabolism in a rat model of EMD.

Methods. Eighty-four male rats weighing 250–300 g were included in the study. EMD was induced by a single subcutaneous injection of 0.18% epinephrine tartrate solution at a dose of 5 mg/kg. Animals received intraperitoneal injections of cryoextract of porcine heart fragments (50 μg of peptides per 100 g of body weight) daily for 14 days. The control group received an equivalent volume of 0.9% sodium chloride solution. The reference group received amiodarone (10 mg/kg). Levels of adenine nucleotuides (ATP, ADP, and AMP) in myocardial tissue were determined using high-performance liquid chromatography, and the myocardial energy charge was calculated.

Results. Treatment with cardiac cryoextract led to a statistically significant increase in ATP content and myocardial energy charge compared to untreated controls. These findings suggest enhanced restoration of cellular energy metabolism and stabilization of the myocardial bioenergetic state under pathological conditions.

Conclusions. The study demonstrated that porcine cardiac cryoextract exhibits pronounced cardioprotective properties by improving myocardial energy processes in experimental myocardial dystrophy. These results support further research to explore its mechanisms of action and potential clinical applications in cardiology.

Keywords: epinephrine-induced myocardial dystrophy, cardiac cryoextract, energy metabolism, adenine nucleotides, energy charge.

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