N 3 (188) 2024. P. 45–51

FEATURES OF THE INTESTINAL MICROBIOME AND THE LEVEL OF LOCAL INFLAMMATORY RESPONSE IN NEWBORNS WITH NEONATAL ENCEPHALOPATHY

S. V. Popov, M. A. Turner, A. O. Profatylo, O. I. Smiian

Sumy State University, Sumy, Ukraine
University of Liverpool, Liverpool, United Kingdom

DOI 10.32782/2226-2008-2024-3-8

Introduction. Hypoxic lesions caused by neonatal encephalopathy (NE) can contribute to disruption of the microbiome formation due to both hypoxia itself and inflammatory reactions. One of the markers for determining the local inflammatory response is fecal calprotectin (FC). It correlates with the level of inflammation and may influence the microbiome composition.

Goal. To study the features of microbiome formation and level of local inflammatory response of newborns with NE by determining the composition of intestinal microbiome and level of FC.

Materials and methods. The study was conducted at 2, 3 and 5 weeks and included 32 controls and 87 newborns with NE. A culture and enzyme immunoassay methods were used to examine the composition of the intestinal microbiome and FC levels. Statistical analyses were performed using SPSS version 28.0.

Results. Newborns with NE had a decrease of levels of Bifidobacterium (p<0.001) and Lactobacilli (p<0.001) at 2 weeks, lower results were noted in severe form of NE. Values of E.coli, Opportunistic pathogens had tendency to increase with higher levels in severe form of NE. FC levels at 2 weeks were increased in children with NE.

Conclusion. Disturbances in the microbiome composition in neonates with NE consisted of a decrease in Bifidobacterium at 2 and 3 weeks, Lactobacilli at 2 weeks and an increase in Opportunistic pathogens at 2 and 3 weeks. In newborns with NE, the level of FC was higher than in controls – for moderate form at 3 and 5 weeks and severe form at 2 and 3 weeks. In newborns with NE received a probiotic, Bifidobacterium values recovered earlier and FC levels were lower.

Key words: neonatal encephalopathy, intestinal microbiome, fecal calprotectin.

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