N 2 (187) 2024. P. 81–84

STUDY OF THE WOUND-HEALING AND REPARATIVE EFFECT OFAN OINTMENT BASED ON THE EXTRACT OF TRIBULUS TERRESTRIS L. ON MODELS OF ASEPTIC FLAT AND LINEAR CUT WOUNDS IN RATS

Ya. V. Rozhkovskyi, S. I. Bohatu, B. V. Prystupa, P. B. Antonenko

Odesa National Medical University, Odesa, Ukraine

DOI 10.32782/2226-2008-2024-2-14

One of the promising ways to improve wound healing is the creation and wider introduction into clinical practice of natural ointments with pronounced anti-inflammatory and reparative properties, which are able to provide a complex therapeutic effect on treating skin wounds.

The aim of the study is to determine the wound-healing and reparative effect of ointment with Tribulus terrestris L. extract in experimental wound damage to the skin in rats.

Materials and methods. In experiments on 80 non-linear white rats of both sexes, it was analyzed the comparative wound-healing and reparative effect of an ointment based on the extract of Tribulus terrestris L., which was created at the Department of General and Clinical Pharmacology and Pharmacognosy of the Odesa National Medical University, and the comparison drug – methyluracil ointment. The comparative wound-healing effect of ointments was studied on the model of an aseptic planar skin wound in rats and evaluated by the planimetric method. The reparative effect of ointments was studied on a model of an aseptic linear incised skin wound and evaluated by the strength of the scar formed during wound healing in rats using the wound tensiometry method. Treatment was carried out by daily one-time application of ointments to the wound surface using an open method.

Results. The property of an ointment based on the extract of Tribulus terrestris L. to shorten the healing time of an aseptic planar skin wound in rats by 1.5–2.0 times was established. In addition, it was shown that in terms of the expressiveness of the wound-healing effect in all terms of the experiment, the ointment under study is not inferior to the comparison drug – methyluracil ointment. In the model of aseptic linear incised skin wound in rats, ointment based on Tribulus terrestris L. extract is superior to methyluracil ointment in terms of reparative activity. The established therapeutic effect of the ointment based on the extract of Tribulus terrestris L. opens up prospects for creating a new ointment based on it for the effective treatment of wound processes of the skin of various genesis.

Key words: ointment with Tribulus terrestris L. extract, methyluracil ointment, wound healing effect, reparative effect.

BIBLIOGRAPHY

  1. Larionov VB, Tsisak AO, Eberle LV, Bieniet SS. Adaptogenic effect of gels with Cetraria islandica L. extract on regenerative properties of skin in a model of thermal burn. Farmakolohiia ta likarska toksykolohiia. 2022; 16(4): 240–247. (in Ukrainian). https://doi.org/10.33250/16.04.240.
  2. Koshova OIu, Myrhorod VS, Bashura OH, Bobro SH, Yeromin OP. Farmakolohichne doslidzhennia kombinovanoho heliu na osnovi ekstraktiv likarskykh roslyn dlia likuvannia dermatoziv. Farmatsevtychnyi chasopys. 2021; (3): 57–63. (in Ukrainian). https://doi.org/10.11603/2312-0967.2021.3.12438.
  3. Maloshtan LM, Zehkhdani EA, Shakina LA. Effect of ointment containing dry licorice root extract on phagocytic activity of blood granulocytic neutrophils in mice with experimental dermatitis. Farmatsevtychnyi chasopys. 2017; (4): 44–47. (in Ukrainian). https://doi.org/10.11603/2312-0967.2016.4.71204.
  4. Maliuhina OO, Smoilovska HP, Bielenichev IF, Mazulin OV, Khortetska TV. Wound healing activity of the lipophilic extract of Tagetes erecta L. Zaporozhye medical journal. 2019; (2): 253–257. Available from: http://zmj.zsmu.edu.ua/article/ view/161510.
  5. Zhu W, Du Y, Meng H, Dong Y, Li L. A review of traditional pharmacological uses, phytochemistry, and pharmacological activities of Tribulus terrestris. Chem Cent J. 2017; 11(1): 60. doi: 10.1186/s13065-017-0289-x.
  6. Ștefănescu R, Tero-Vescan A, Negroiu A, Aurică E, Vari CE. AComprehensive Review of the Phytochemical, Pharmacological, and Toxicological Properties of Tribulus terrestris L. Biomolecules. 2020; 10(5): 752. doi: 10.3390/biom10050752.
  7. Tian C, Chang Y, Zhang Z, et al. Extraction technology, component analysis, antioxidant, antibacterial, analgesic and anti-inflammatory activities of flavonoids fraction from Tribulus terrestris leaves. Heliyon. 2019; 5(8): e02234. Published 2019 Aug 22. doi: 10.1016/j.heliyon.2019.e02234.
  8. Khalid A, Nadeem T, Khan MA, Ali Q, Zubair M. In vitro evaluation of immunomodulatory, anti-diabetic, and anti-cancer molecular mechanisms of Tribulus terrestris extracts. Sci Rep. 2022; 12(1): 22478. doi: 10.1038/s41598-022-26742-6. PMID: 36577761; PMCID: PMC9797551.
  9. Ghildiyal S, Gautam MK, Joshi VK, Goel RK. Wound healing and antimicrobial activity of two classical formulations of Laghupanchamula in rats. J Ayurveda Integr Med. 2015; 6(4): 241–7. doi: 10.4103/0975-9476.157952. PMID: 26834423; PMCID: PMC4719484.
  10. Burda NYe, Klyvniak BМ, Zhuravel IO.; National Pharmaceutical University. Medical agents with anti-inflammatory action: pat. 110212 UA. 2016 December 26. 18. 3 p. (in Ukrainian).
  11. Klyvniak BМ. Pharmacognostic study of Tribulus terrestris L. (dissertation). Odesa: Odesa National Medical University; 2017. 139 p. (in Ukrainian).
  12. Yakovlieva LV, Tkachova OV, Butko YaO, Larianovska YuB. Experimental study of new drugs for local treatment of wounds. Guidelines. Kharkiv: NfaU; 2013. 52 p. (in Ukrainian).
  13. Stefanov OV. Preclinical research of medicines: method. recommendations. Kyiv: Avitsena; 2001. 528 p. (in Ukrainian).
View article PDF