N 2 (187) 2024. P. 75–80

BIOLOGICAL ACTIVITY OF 3-HYDROXYMETHYLPYRIDINIUM HEXAFLUOROSILICATE MONOHYDRATE AND 4-HYDROXYMETHYLPYRIDINIUM HEXAFLUOROSILICATE AS CANDIDATES FOR ANTICARIES AGENTS

V. O. Gelmboldt, L. M. Khromagina, O. V. Nikitin, N. S. Fizor

Odessa National Medical University, Odesa, Ukraine,

State Institution “Institute of Stomatology and Maxillofacial Surgery of the National Academy of Medical Sciences of Ukraine”, Odesa, Ukraine

DOI 10.32782/2226-2008-2024-2-13

The aim of the study. Determination of the biological activity of new compounds – 3-hydroxymethylpyridinium hexafluorosilicate monohydrate (I) and 4-hydroxymethylpyridinium hexafluorosilicate (II), which we synthesized earlier.

Materials and methods. The experiments were carried out on 42 white male Wistar rats, divided into 7 equal groups; working groups received Stefan’s cariogenic diet. Fluorine-containing salts were used in the composition of gels, with dose of fluorine of 1.00 mg/kg. All rats (except Sundays) had applications of gels, covering the teeth and gums. Reference drugs – sodium fluoride and (NH4)2SiF6.

Results and discussion. The caries-preventive efficacy (CPE) of fluorine-containing compounds was calculated. The activity of acid and alkaline phosphatases was determined in the incisor pulp homogenate, and the levels of malondialdehyde, elastase, and lysozyme were determined in the homogenate of the oral mucosa. Alanine aminotransferase activity was determined in blood serum. The CPE value for II is 41.5%, which is 1.4 times greater than NaF. A significant positive effect of fluoride agents on the biochemical parameters of the dental pulp and oral mucosa of rats (mineralizing index, elastase, urease and lysozyme activities) that received a cariogenic diet was established. Hexafluorosilicates more effectively normalize biochemical parameters compared to the action of sodium fluoride in the absence of hepatotoxic effects for all the studied compounds.

Key words: pyridinium hexafluorosilicates, caries-preventive efficacy, mineralizing index, activity of alanine aminotransferase, level of malondialdehyde.

BIBLIOGRAPHY

  1. Pitts NB, Zero DT, Marsh PD. et al. Dental caries. Rev. Dis. Primers. 2017; 3: 17030. https://doi.org/10.1038/ nrdp.2017.30.
  2. Sharkov N. Effects of nicomethanol hydrofluoride on dental enamel and synthetic apatites: a role for anti-caries protection. J. Paediatr. Dent. 2017; 18: 411–418. https://doi.org/10.1007/s40368-017-0314-8.
  3. Gelmboldt VO, Kravtsov VCh, Fonari MS. Ammonium hexafluoridosilicates: Synthesis, structures, properties, applications. Fluor. Chem. 2019; 221: 91–102. https://doi.org/10.1016/j.jfluchem.2019.04.005.
  4. Gelmboldt VО, Anisimov VYu, Shyshkin IO, Fonari MS, Kravtsov VCh. Synthesis, crystal structures, properties and caries prevention efficiency of 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates. Fluor. Chem. 2018; 205: 15–21. https:// doi.org/10.1016/j.jfluchem.2017.11.004.
  5. GelmboldtVO, Lytvynchuk IV, Shyshkin IO, Khromagina LN, KravtsovVCh, Fonari MS. Bis(2-, 3-, 4-carboxyethylpyridinium) hexafluorosilicates as potential caries prophylactic agents. Pharm. 2022; 335(7): e2200074. https://doi.org/10.1002/ ardp.202200074.
  6. Shyshkin IO, Nikitin OV, Gelmboldt VO. Identification of ammonium hexafluorosilicates using chemical methods of analysis. Odesa Med. J. 2023; 4(185): 94–98. (in Ukrainian). https://doi.org/10.32782/2226-2008-2023-4-18.
  7. Gelmboldt VO, Shyshkin IO, Anisimov VYu, Fonari MS, Kravtsov VCh. Bis(3-hydroxymethylpyridinium) hexafluorosilicate monohydrate as a new potential anticaries agent: Synthesis, crystal structure and pharmacological properties. Fluor. Chem. 2020; 235: 109547. https://doi.org/10.1016/j.jfluchem.2020.109547.
  8. Lepskiy VV, Anisimov VYu, Prodan OV, Gelmboldt VO. Experimental evaluation of caries preventive efficiency “onium” hexafluorosilicates. Visnyk stomatologii. 2015; 2: 10–13 (in Russian).
  9. Stephan RM, Harris MR. Location of experimental caries on different tooth surfaces in the Norway rat, in: R.F. Sognnaes (Ed.), Advances in Experimental Caries Research. Washington: American Association for the Advancement of Science, 1955; 47–65.
  10. Makarenko OA, Khromagina LM, Khodakov IV, Maikova HV, Mudryk LM, Kika VV, Mogilevska TV. Methods of researching the state of intestines and bones in laboratory rats. Directory. Odesa: publisher S.L. Nazarchuk, 2022; 81 p. (in Ukrainian).
  11. Goryachkovsky AM. Clinical biochemistry in laboratory diagnostics. Odessa: Ecology, 2005; 616 p. (in Russian).
  12. Levitsky AP, Makarenko OA, Khodakov IV, Zelenina YuV. Enzymatic method of the estimation of bone tissue state. Odesa Med. J. 2006; 3: 17–21 (in Ukrainian).
  13. Levitsky AP. Therapeutic and prophylactic dental elixirs: a tutorial. Odessa: KP OGT, 2010; 258 p. (in Russian).
  14. Prylutskyi YuI, Ilchenko OV, Tsymbalyuk OV, Kosterin SO. Statistical methods in biology: tutorial. Kyiv: Nauk. dumka, 2017. 216 p.
  15. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Med. Cell. Longev. 2014. Article ID 360438. http://dx.doi.org/10.1155/2014/360438.
  16. Kobayashi A, Suzuki Yu, Sugai S. Specificity of transaminase activities in the prediction of drug-induced hepatotoxicity. J. Toxicol. Sci. 2020; 45(9): 515–537. https://doi.org/10.2131/jts.45.515.
View article PDF