N 2 (187) 2024. P. 19–23

PECULIARITIES OF BRAIN ANGIOGENESIS UNDER PENTYLENETETRAZOLE (PTZ)-INDUCED KINDLING AND CEREBELLAR TRANSCRANIAL DIRECT CURRENT STIMULATION

M. P. Pervak, N. I. Bukreeva, O. A. Kashchenko, O. D. Ryabenka,
L. S. Godlevsky

Odesa National Medical University, Odesa, Ukraine

DOI 10.32782/2226-2008-2024-2-3

Background. Angiogenesis associated with neuroimmune inflammation is an important pathogenetic mechanism of chronic epileptogenesis.

The study aimed to study the microvascular density in the brain cortex of rats with pentylenetetrazol (PTZ)-induced kindling and under transcranial direct current stimulation conditions.

Methods. For three weeks, kindling was produced via PTZ (35,0 mg/kg, i.p.) administration. TDCS (500 μA, 15.0 min) was applied with a cathode to the frontal and cerebellar cortex 60 min before each subsequent administration of an epileptogen.

Results. Cerebellar TDCS delays the development of generalized convulsions, while frontal cortex TDCS causes a less pronounced protective effect. The density of microvessels, which was determined by the presence of growth buds and endothelial cell mitoses in rats with developed kindling, increased by 47.6% compared to the control (P<0.05). Against the background of cerebellar TDCS, the density of microvessels exceeded that in the control group by 10,4% (P>0.05) and was significantly lower compared to the group with false stimulations (P<0.05). The microvascular density determined by collagen IV staining in rats with developed kindling was 63.1% higher than in the control group (P<0.05). In kindling rats with TDCS, such differences were 25.7% (P<0.05) and, at the same time, were significantly lower (by 23.0%) compared to that in kindling rats with false TDCS (P<0.05).

Conclusions. The cerebellar cathode-triggered TDCS prevents the formation of kindling-induced seizures and angiogenesis in the cerebral cortex. Moreover, it reduces endothelial cell mitosis and collagen IV production.

Key words: chronic epileptic activity, transcranial direct current stimulation, cerebellum, angiogenesis, collagen IV.

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