N 4 (185) 2023. P. 75–82

CELL-FREE BIOLOGICS: FOCUS ON MESENCHYMAL STEM CELL CONDITIONED MEDIA

F. V. Hladkykh

N. Karazin Kharkiv National University, Kharkiv, Ukraine
State Organization “Grigoriev Institute for Medical Radiology and Oncology of the National Academy of Medical Sciences of Ukraine”, Kharkiv, Ukraine
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

DOI 10.32782/2226-2008-2023-4-15

Introduction. The term “conditioned medium” refers to the liquid phase of the cell culture medium enriched with the secretome of the cultured tissue. The culture medium enriched with the secretome from mesenchymal stem cells (MSCs) during their growth was named MSC conditioned medium (MSC-CM).

Objective. Summarize the current information on the conditioned medium of mesenchymal stem cells as an innovative cell-free biological tool according to open source data.

Methods. Publications were selected based on PubMed, Clinical Key Elsevier, Cochrane Library, eBook Business Collection, and Google Scholar databases, which covered information on the use of MSC-CM as innovative cell-free biological agents.

Results. The paradigm shift regarding the mode of action of MSCs contributed to the formation of the concept of cellular secretion, both as a whole concept and as separate fractions, and to a new class of biological therapeutic agents. In recent years, the secret of MSCs has been repeating the path of clinical application of donor cells. MSC secretome is a mixture of biologically active vesicles and individual lipids, proteins and nucleic acids dissolved in the liquid phase. The characteristics of MSC-CM vary depending on the actual source of initial MSCs, gas conditions of cultivation – normoxia or hypoxia, duration of cultivation, etc. The culture medium in in vitro culture represents the microenvironment in vivo and can determine cell fate and thus paracrine properties.

Conclusions. The use of MSC-CM has advantages over MSC itself due to the lack of immunogenicity, which allows minimizing inter- donor variability and avoiding the need to perform additional procedures in patients for collecting cells.

Key words: cell-free biological agents, conditioned medium, extracellular vesicles, exosomes, culture medium.

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