Danylo Halytsky Lviv National Medical University, Ukraine
DOI 10.32782/2226-2008-2023-4-3
The pathogenesis of periodontitis mainly involves the disease-associated oral microbiota, inflammation of the body and environmental and genetic risk factors. It has been established that the immune reactions of the human immune system determine susceptibility to periodontal disease. However, the precise role of different immune cells in periodontitis, the role of immunity in alveolar bone destruction, and the specific signaling pathways involved in immune regulation in periodontitis remain obscure.
The aim of the study is to establish the state of individual components of the humoral and cellular links of immunity in the guinea pigs’ blood with experimental periodontitis.
Research materials and methods. Experimental studies were conducted on 46 guinea pigs (males) weighing 180–220 g, divided into 5 groups of 9 animals in each except for the first (10 animals).
The model of experimental periodontitis was reproduced following the method of O. N. Voskresensky, determination of the content of T- and B-lymphocytes in the blood – following the method of Chernushenko E. F., Kogosova L. S., determination of circulating immune complexes in blood – following the method of V. V. Menshikova.
Statistical processing of the obtained data was carried out through the Student’s t-test.
The obtained results showed a significant decrease in the number of T-lymphocytes for all days of the experimental simulation of the disease against the background of an increase in the content of B-lymphocytes and circulating immune complexes which indicates the depression of the cellular mechanisms of the body’s defense and the activation of the humoral link of the immune response during the development of experimental periodontitis.
Key words: experimental periodontitis, T-lymphocytes, B-lymphocytes, circulating immune complexes.
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