N 3 (184) 2023. P. 9–11

STUDY OF BIOCHEMICAL INDICES OF BLOOD SERUM OF EXPERIMENTAL ANIMALS IN MODELING THE ABNORMAL TIMING OF DENTITION

V. V. Horokhovskyi, O. V. Denga

Odesa National Medical University, Odesa, Ukraine
State Institution “Institute of Dentistry and Maxillofacial Surgery of the National Academy of Medical Sciences of Ukraine”, Odesa, Ukraine

DOI 10.32782/2226-2008-2023-3-1

The studies are dedicated to the investigation of changes in biochemical markers (alkaline phosphatase, elastase and catalase) in the blood serum of rats when simulating the abnormal timing of dentition.

Materials and methods of research. The experiment was performed on 20 white female laboratory rats and 37 male rats. Starting from the first day of drug administration, the males were put together with females. Depending on the drugs used, the animals were divided into 4 groups: 1. Intact (vivarium diet); 2. L-thyroxine in a dose of 10 mg/kg + vivarium diet; 3. Antibiotics (cefoperazone 180 mg/kg – pregnancy, amoxiclav 135 mg/kg – lactation) + vivarium diet; 4. Mercazolil – (20 mg/kg – pregnancy), (50 mg/kg – lactation) + vivarium diet. Further studies were conducted on little rat that were born from the females receiving these drugs. Rats were removed from the experiment under thiopental anesthesia (20 mg/kg) after the lactation period at approximately 35 days of age. The total duration of the experiment was 56 days. A biochemical analysis of the blood serum was performed in rats of all groups.

Research results and their discussion. The results of the studies indicate the development of systemic inflammation after taking L-thyroxine and antibiotics, which is confirmed by the activity of elastase in the blood serum of rats. Inhibition of the phagocytic activity of neutrophils in rats of the 4th group, which were obtained from the females that had a deficiency of thyroid hormones during pregnancy and lactation, was proved. There was revealed suppression of the activity of the blood antioxidant system after the use of thyroxine and its stimulation after receiving antibiotics or mercazolil.

Key words: timing of dentition, biochemistry, rats.

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