Aim. To study gene polymorphisms of IL-10 (G1082A), IL-4 (C589T) and TNF-α (G308A) and cellular immunity (CD3 +, CD4 +, CD8 +, CD16 +, CD19 +) in patients with chronic hepatitis В (CHВ) and healthy persons belonging to ethnically homogeneous group of residents of the Odessa region, as well as identifying possible association of certain genotypes and the degree of liver fibrosis.
Materials and methods. The study includes 41 patients with CHВ. The control group includes of 30 people who did not have diseases of the hepatobiliary system. Traditional diagnostic methods were used by the provided protocol. Polymorphism amplification was investigated using respective portions of the genome by PCR. The structure of the primers used, are described in the GenBank. Evaluation of morphological changes in the liver (the degree of activity and severity of fibrotic changes) was determined on a scale METAVIR using a non-invasive method Fibrotest.
Results. It is established a certain level of statistical significance of certain genotypes of cytokines, cellular immunity and the degree of liver fibrosis. Patients with homozygous CC genotype IL-4 had low level of fibrotic changes in the liver than in carriers of the heterozygous СT genotype (p<0.01).
Patients with homozygous genotype GG TNF-α had low level of liver fibrosis than in carriers of the heterozygous genotype GA (p<0.01). Patients with smaller changes in cellular immunity (reduction of CD3+, CD4+, CD16+, CD19+) had low level of liver fibrosis (p<0.01).
Conclusion. It is established that degree of liver fibrosis and certain genotypes IL-4 and TNF-α allows to use the information as one criterion for the speed of progression of liver fibrotic processes. Pronounced changes in cellular immunity is an additional criterion for the degree of morphological disturbances in liver tissue.