Purpose of research: to explore the possibility of associations of genetic markers with peculiarities of development and clinical course of osteoarthrosis of knee joints in women in the menopausal period.
Materials and methods. We examined 120 women who were the main group diagnosed with knee osteoarthrosis according to the criteria of the American College of Rheumatologists (ASRs) having the I–II radiological stage for Kellgren-Lawrence, the menopausal period (the mean duration of menopause was (8.50±0.43) years old) aged 50 to 70 years. The average duration of the disease is (7.02±1.96) years. All patients of the main group performed ultrasound knee joints for the presence of synovitis. All patients were consulted by the endocrinologist and gynecologist. The control group consisted of 100 women of the same age, but without clinical manifestations of osteoarthritis of the knee joints. Clinical and genetic studies included the conduct of molecular genetic analysis with the definition of mutations of genes: collagen 1 — Со1а1 С/А; vitamin D receptor — VDR T352C; estrogens — ER: Pvull and ER: Khbal; interleukin 1 — IL1A1 T4845G; tumor necrosis factor –TNF-308G/A; matrix metalloproteinase — MMP1-1607insG in buccal epithelium cells. Polymorphisms of genes were evaluated by PCR.
Molecular genetic methods of research were carried out in the genetic laboratory “GERMEDTEH” in Odessa.
Results. The presence of associations of T/C alleles of the VDR T352C gene, alleles T/T (χ2=26,1; RR=2,25), G/G (χ2=23,1; RR=2,6) of the IL1A1 gene and G alleles / G (χ2=7.5; RR=2.6) of the TNF-308G/A gene, of the C/C alleles of the Со11A1 gene (χ2=8.1; RR=2.7); alleles T/T (χ2=261, RR=2.25) and G/G (χ2=23.1; RR=2.03) of the IL1A1 gene and G/G alleles (χ2=7.5, RR=2.6) and the TNF-308G/A may be associated with the involvement of these alleles products in the pathogenesis of OA. There are certain differences in the distribution of gene alleles in women with OA with different clinical course, namely, the characteristics of pain syndrome and the presence of inflammatory reactions. Pain syndrome may be due to the rate of bone remodeling, the presence of inflammatory reactions that may be associated with a violation of the metabolism of inflammatory mediators.