Adolescence is decisive with regard to the formation of reproductive function, so almost in half of cases childhood and adolescence diseases are causes of male infertility. To date no emphasis has been placed on examination of effect of childhood metabolic syndrome (MS) on testes parameters in puberty.
The aim of present study was an estimation of biochemical indices in rat testes following MS induced in juvenile age.
Wistar albino male rats of 21 days age were divided into 2 groups (10 animals in each): (1) control, (2) MS. MS was induced by full replacement of drinking water by 10 % fructose solution. To confirm the adequacy of the applied model of MC, the relevant clinical biochemical parameters of blood serum were evaluated. It was shown an increase in the glucose content by 71 %, triglycerides by 43 %, total cholesterol by almost 20 %, and low density lipoprotein content by 36 % as compared with control. Development of MS in childhood greatly affected testicular pro- and antioxidant systems: we recorded lipid peroxidation processes intensification 2.2 times, increase of catalase activity 15 % and decrease of glutathione content 9 % as compared with control.
Changes in the activity of testicular glutathione-dependent enzymes have not been revealed at this stage of the pathological process.
Thus, induction of MS in childhood provoked remote disturbances in testicular cells at biochemical level. These and other pathological events in testicular tissues need to be investigated profoundly as processes affecting spermatogenesis and probably underlying male infertility in adults. The results substantiate further studies, including the development of effective methods of prevention and treatment of male infertility caused by MS.