Introduction. Herpetic lesions of the eye is a major cause of infectious corneal blindness. Viral lesion of eyeball surface often has chronic process. Using of traditional drugs does not always provide a positive therapeutic effect and prevents recurrence, determining the relevance of new approaches to the treatment of viral pathologies of the cornea.
Objective. To study the effect of silver nanoparticles in vitro on the expression of molecular markers of activation of lymphocytes and the phagocytic activity of neutrophils in patients with herpetic keratitis.
Materials and methods. The research was conducted with 33 patient with herpetic keratitis. It was in vitro investigated the expression of molecular markers of activation of lymphocytes (CD 5, CD7, CD25, CD38, CD45, CD54, CD95, CD150) peripheral blood and phagocytic activity of neutrophils before and after cultivation with a colloidal solution of 30 nm silver nanoparticles.
Results. After cultivation with silver nanoparticles the expression of molecular markers CD7 increased from (21.1±2.6) up to (27.4±1.7)%; CD25 from (19.2±1.1) up to (26.8±1.2) and CD45 from (22.3±1.1) up to (31.1±1.8)% (p<0.05). It was not found silver nanoparticles influence on the expression of markers СD5, CD38, CD54, CD95, CD150. After cultivation with silver nanoparticles phagocytic activity of neutrophils increase from (58.5±3.9)% up to (79.4±4.1)% (p<0.05).
Conclusion. Silver nanoparticles of 30 nm in vitro cause the change of the functional activity of leukocytes and lymphocytes in patients with herpetic keratitis, as reflected in the increasing of lymphocyte activation markers expression CD7, CD25, CD45 and phagocytic activity of neutrophils.