Aim: On the basis of multivariate analysis of indicators of cellular immunity Rh sensitized women to identify the most important laboratory markers and their association.
Materials and methods. We conducted a study of cellular immunity (populations and subpopulations of lymphocytes) in 37 Rh-sensitized women with different antibody titers observed and delivered in Maternity hospital № 7 (Odesa).
Results. It may be noted according to the results of multivariate analysis with rotation Varimax raw that the factor 1 was activated subpopulation of T cells and T-killer cells. With the direct dependence are: CD3+HLA-DR+ (activated T cells) with correlation coefficient 0.84, CD3+ CD4+HLA-DR+ (activated T-helpers cells) with a coefficient of 0,83, CD3+CD8+HLA-DR+ (activated T-suppressors) with the same correlation of 0.83. With inverse correlation in the group represented by NK-cells CD3-CD16/56+ (T-killers), the coupling coefficient is equal to 0.77. Factor 2 showed by indicators of the population of B-lymphocytes (CD19+) with a direct correlation of 0.88, and T-lymphocytes (CD3+) with an inverse relationship 0.92. Factor 3 includes main subpopulations of lymphocytes (T-helpers and T-suppressors) and immunoregulatory index. CD3+CD8+ (T-suppressors) ratio of 0.69 are presented by the direct connection. CD3+CD4+ (T-helpers) — 0.95 immunoregulatory index and CD3+CD4+/CD3+CD8+ with a correlation coefficient — 0.78 are presented by the negative connection.
Conclusion. The results of our studies showed that the most interesting indicaors for studying the patterns of cellular immunity in Rh-sensitized women are: CD19+ (B-lymphocytes), CD3+ (T-lymphocytes), CD3+HLA-DR+ (activated T-lymphocytes), CD3+CD4+HLA-DR+ (activated T-helpers), CD3+CD8+HLA-DR+ (activated T-suppressors), NK-cells CD3-CD16/56 (T-killers) and immunoregulatory index CD3+CD4+/CD3+CD8+.