Background. The main thing in the system of preventive measures directed to removal of harmful consequences of chemistries of national economy is an observance of acceptable levels of chemicals influence. In realization of this problem the leading role is played by the complex of toxicological-hygienic researches by a few stages, one of which is revealing of features of the toxic impact on an organism.
Methods. The clean stadards of AMINF are in-process used chemically and its derivative AMINF4. Level of ascorbic acid in the liver of rats was determined by the Tilman method. Activity of katalase in blood was estimated by spectrophotometer at 230 nm after the decline of peroxigen in an incubation environment. The level of malonic dialdehyde in the serum of blood was estimated colorimetrically by realization of reaction between MDA and thiobarbituric acid with formation of the painted thremetin complex with a maximum of absorption at 532 nm.
Results. Results testified to some increase (p<0.05) when compared to control of content of ascorbic acid in the liver of rats at the 10 and 20th day of actions of substances in a dose 1/10 LD50, while at the 30th day — decline (p<0.05). Dynamics of changes of content of this index at peroral introduction to the experimental rats of AMINF and MINF4 in a dose 1/100 LD50 was another there an increase (p<0.05) of level in all terms of supervision, most pronounced — at the 20th day.
Conclusions. It is well-proven that the probed chemical substances in a dose 1/10 Ld50 cause a decline of ascorbic acid level in the liver of rats, since the 20th day of administation, which testifies from one side about the decline of its synthesis, and from the other — about exhaustion of its participating in the antioxidant system of organism. The dose 1/100 Ld50 of substance coused increase of ascorbic acid level during 30 days of administration, which represents strengthening of its synthesis for rats and forming protective reaction to xenobiotics administration.