Development of methods of extraction in vitro and in vivo 14С-2-(β-diethyloamino)- ethylmercapto-5-izopropyl-3,4-dihydropyrimidine-6-on-4-hydrochloride (I) and 14С-5-izopropyl-2-thiobarbituric acids (II) is carried out. On the basis of metrological characteristics of the extraction process optimum conditions (рН and a ratio of bioassay and organic solvent) substance extraction have been studied. the radiochromatografic and mass-spectrometer analysis has shown that metabolites of 14С-I are thiobarbituric acids and product of its biotransformation — 5-izopropylbarbituric acids.
