Introduction: Article is devoted to the improvement of immunoprevention of diphtheria as for development of the complex candidate vaccines made on the basis of the native diphtherial bacterial antigens obtained by the physical factors in the conditions of diphtherial production.
The aim and purposes of the study was to reproduce the process of obtaining diphtheria bacterial antigen under production conditions of PJSC “Pharmstandard-Biolek” and determine the specific activity of the experimental samples of complex diphtheria candidate vaccines produced on its basis.
Methods: The diphtheria bacterial antigen preparation was obtained by exclusive method and used for immunization of laboratory rabbits with the native cleared diphtherial toxoid. As a control there was taken AD-M vaccine. Subsequently, serums were examined in RPHA.
Results. Both series of experimental diphtherial bacterial preparations which differed in concentration of protein and have been received by means of various sources of ultrasonic radiation, combined with the native cleared diphtherial toxoid, have shown ability to effectively stimulate anti-toxic humoral immunity of experimental animals.
Conclusions. The reached levels of antidiphtherial antibodies in RPHA after inoculation of the studied samples of candidate-vaccines with a bacterial component to experimental animals were comparable to action of an AD-M vaccine where as adjuvan aluminum hydroxide is used.