Aim: To determine and compare the therapeutic and preventive effects of antidysbiotic formulations (ADF) lequin and lysozyme, respectively, on the state of salivary glands at nonalcoholic steatohepatitis (NASH). Lequin is the multifunctional formulation which includes hepatoprotector lecithin, bioflavonoid quercetin, prebiotic inulin and calcium citrate.
Materials and methods. Experiments were performed at 40 white Wistar rats. In 30 rats the experimental NASH was induced by feeding them with a high fat diet (fodder plus 15% of sunflower oil) and drinking water with lincomycin at a dose of 70 mg/kg. The ADF lequin was administered per os at a dose of 300 mg/kg /day for 20 days, preparate of lysozyme introduced per os in dose 30 mg/kg. The content of malondialdehyde (MDA), the activity of elastase, urease, lysozyme and catalase were determined in the homogenates of the parotid and submandibular glands. The degree of dysbiosis by Levitsky was calculated as the ratio of the relative activities of urease and lysozyme; the antioxidant-prooxidant index (API) was calculated as the ratio of the catalase activity and MDA content.
Results. At NASH in the salivary glands the levels of MDA, urease, and the degree of dysbiosis are increased. The activities of lysozyme, catalase, and the level of API are decreased. Taking ADF reduced levels of MDA, urease, degree of dysbiosis and raised levels of lysozyme, catalase and API index. In its therapeutic and prophylactic effects lequin is preferable as compared to lysozyme.
Conclusion. NASH induces the development of dysbiosis, inflammation and the reduction of levels of antioxidant defenses in the salivary glands. ADF lequin provides therapeutic and prophylactic effects, surpassing the number of indicators for lysozyme.