Introduction. Exogenous allergic alveolitis (AA) leads to such complications as fibrosis, respiratory failure, causes invalidity and temporary or permanent disability, especially during the abnormality of coronary blood circulation. As for today, pathogenesis of AA is not studied to the end, particularly the role of free radical oxidation (FRO) processes and status of antioxidant protection (AOP) in the mechanisms of the disease. Also, the mechanisms of AA formations during adrenal myocardial damage (AMD) remain unclear. Effect of tiotriazoline on markers of lipid peroxidation (LPO) and antioxidant system (AOS) in conditions of these two pathologies combined in the experiment is not established.
Purpose. That is why the purpose of our study was to determine the effect of tiotriazoline to the indicators of prooxidant and antioxidant systems in blood during AA and AMD.
Materials and methods. Experiments were carried out on 68 guinea pigs (male) weighing 0.18–0.22 kg. The animals were divided into six groups. First group was the intact animals. The second and third groups consisted of guinea pigs with EAA on the 1st and 7th day of the experiment respectively. The fourth and fifth groups consisted of guinea pigs which were examined on the 14th and 24th day of this experimental disease model. The sixth group is guinea pigs with EAA and AMD after tiotriazoline intramuscular injections once a day at a dose of 100 mg per 1 kg and during 10 days (from 14th to 24th day).
Results and discussion. The results showed that in the dynamics (1st, 7th, 14th, 24th day of allergic alveolitis and AMD) there is gradual increase of content of diene conjugates (DC) and malondialdehyde (MDA) in blood, which indicates a strengthening of free radical oxidation.
Determination of superoxide dismutase and catalase levels made it possible to establish non-unidirectional changes. So on the 1st and 7th days of AA and AMD activity of these enzymes in the blood was increased, and then, on the 14th and 24th days of the experiment, enzymes acquired the opposing direction of changes. The results of these enzymes experiments showed, that on the 1st and 7th days of AA and AMD there is a compensatory response of AOS, and then, on the 14th and 24th days it significantly depletes, which indicates depression.
Application of tiotriazoline caused decreased content of diene conjugates and MDA in blood and increased activity of SOD and CT in the group of animals with AA and AMD, which were not been injected by this medicine.
Conclusions. Thus, carried out biochemical studies make it possible to reveal a part of the one of important molecular mechanisms of cell damage and establish corrective influence on tiotriazoline to the altered markers of FRO and AOS in blood while AA and AMD.