The negative effect of methotrexate on the immune and hematopoietic systems complicates its use in long-term courses and can reduce the effectiveness of anticancer therapy.
Thus, the aim of our work was pharmacological study of the combination of glucosamine derivatives with quercetin as a potential corrector of toxic effects on the blood and immunodepressive action of methotrexate in the experiment on rats.
Materials and methods. Protective effect on the hematopoietic and immune systems of the combination was evaluated by the following parameters: peripheral blood, weighting coefficients of the thymus, spleen, liver and the results of the morphological study of their tissues.
Results and discussion. It was confirmed the toxic effect on the blood and immunosuppressive action of methotrexate. Introduction of the combination of glucosamine derivatives with quercetin produced a positive effect on the structural and functional state of the blood-forming organs at conditions of methotrexate-induced pathology. It was expressed certain positive changes of the parameters on peripheral blood relative to the control group animals, normalization of the weighting coefficients of the thymus, spleen, liver, and also improved the morphological pattern of these organs. Summary data of the experiment determined that the majority of the studied parameters of the combination of glucosamine derivatives with quercetin exerted an expressive protective effect than the referent remedy quercetin.
Conclusions. Obtained data determine the prospects of further study combination of glucosamine derivatives with quercetin as means for modification of toxic effects of antimetabolite drugs.