Introduction. Cirrhosis is a chronic liver disease characterized by diffuse changes in its parenchyma in form of hepatocellular fibrosis, leading to the development of portal hypertension and liver failure. Platelet-rich plasma (PRP) is the platelet concentrate in a small volume of plasma where content of cytokines and regulatory proteins is significantly increased.
The aim of research. The research was devoted to evaluate the effectiveness of platelet-rich plasma in experimental therapy of liver cirrhosis in rats.
Methods. To create the model of cirrhosis the rat males got the motor and olive oil at a concentration of 50% at a dose of 0.4 ml into the stomach every 3rd day during 16 weeks, and drunk water solution of ethanol at a concentration of 10% ad libidum. To obtain PRP from the whole blood the unit SmartPrep (Harvester Corp.,USA) was used. The animals got 2 injections of PRP with an interval of 2 weeks.
Results and discussion. Double administration of PRP in liver tissue in case of cirrhosis, reduces the amount of connective tissue and the number of collagen fibers at the place of necrosis due to hepatic cirrhosis; an active regeneration of liver tissue microstructure with restoration of its microstructure exists. A number of PAS-positive substances was highly increased in comparison with the group without correction. The results, probably, were obtained due to the action of cytokines such as endothelial growth factor, vascular endothelial growth factor, fibroblast growth factor etc., which are released from platelets when administered to the organ. In the animals without treatment the active process of outgrowing of connective tissue was found.
Conclusions. The effectiveness of PRP treatment of hepatic cirrhosis in rats is high. No adverse reactions were observed. PRP is a mixture of autologous cells, it is physiological to the body, and does not require immunosuppression and special conditions for treatment as it usually happens in the transplantation of heterogeneous material.