Background. Discovering of therapeutic effect of silver nanoparticles (NP) is an important question of research in the field of nanomedicine. The available literature data on the effect on the blood vessels of the skin AgNP contain conflicting information. Published data indicate a size-depended effects on the blood vessels of the skin and the need for a detailed study of the nature of this effect depending on the size of nanoparticles.
Objective. To investigate the morphometric changes in the skin vesels tissues that occur during intradermal administration of silver nanoparticles.
Methods. Spherical silver nanoparticles 30 nm were used. The experiment was carried out on 140 Wistar rats. Animals were divided into 4 groups: intact animals, animals injected with silver nanoparticles, Protargolum, saline. After subcutaneous administration of 0.01 ml solution effect was assessed at 1st, 3rd, 7th, 14th, 21st, 30th, 45th day. Standard histological techniques were used.
Results. In rat skin the vessels form several textures, which are located under layered structure of the skin. Between the papillae and mesh layer there located subpapilar plexus capillaries which form a loop. On the border of the mesh layer and hypodermis lies subdermal plexus, and hypodermal plexus locates under hypodermis. It is shown that the introduction of silver nanoparticles in the skin provokes pronounced expansion of the subpapilar vascular plexus and an increase in the specific area of the vessels, which lasts up to 21 days. The restoration of the vascular bed to the original value occurs on the 45th day. In the Protargolum group it is also noted an increase in the size of blood vessels, but there is no increase in the specific area of vascular loops. This process does not spread to the surrounding area.
Conclusion. Introduction of silver nanoparticles 30 nm leads to pronounced expansion of the skin vascular plexus