Introduction. Mucoadhesive films, containing biologically active substance, have some advantages over the other dosage forms, because they provide long-term local action of medicinal means with usage of minimal quantity of acting substance. Formerly we had shown the effectiveness of mucaodhesive films (MAF) with immobilized lysozyme usage, possessing the antiinflammatory and antibacterial effect on the rats oral cavity mucous membrane (OCMM) after application of bee venom.
Materials and methods. Experiments were conducted on Wistar rats. In the homogenates of OCMM the elastase activity, malonic dialdehyde and hyaluronic acid content, urease, lysozyme and catalase activity were measured; the disbiosis and antioxidative level were determined.
The results of investigation were mathematically treated.
Results and conclusions. The medicobiologic properties of MAF based on polyvinyl alcohol cryogel with immobilized lysozyme and preservatives of complex action were investigated. It was shown that application of lipopolysaccharide on oral cavity mucous membranes induced inflammation and disbiosis, decreasing antioxidant defence and hyaluronic acid level. It was established that MAF with entraped lysozyme, chlorhexidine bigluconate and ethylenediamine tetraacetate promotes 50% decrease of urease level-marker of microbial dissemination of rats cheek’s mucous membrane. Application on rats’ OCMM of lysozyme-containing films increases the hyaluronic acid content and decreases both disbiosis and inflammation degree.