This study introduces nanoparticles comprising a magnetic core and a silver surface layer, which are recognized as a promising candidate to fight against antibiotic resistant pathogens in patients receiving critical care treatment.
The objective of the work presented was to explore the influence of core & shell-type nanoparticles FeFe2O4&Ag0 upon a phagocytic component of immune system during experimental peritonitis.
Materials and methods. Phagocytic cells from peritoneal lavage fluid of C57Bl mice was examined using Rosein staining, Trypan blue exclusion test, and Chemiluminescent analysis of respiratory burst, 4 and 24 hours after an aseptic injection of zymosan alone or with anticipatory introduction of the nanocomposite.
Results and discussion. The injection of zymosan was followed by the increase in both mononuclear phagocytes and polymorphonuclear leukocytes recruitment into peritoneal cavity. The phagocytic cells of peritoneal lavage fluid had less viability and were highly activated in comparison with intact animals. The introduction of silver&magnetite nanocomposite had significantly prevented polymorphonuclear leukocytes infiltration though augmenting mononuclear phagocytes content. In later term after the nanodrug using a decrease in the rate of reactive oxygen species production was revealed, while the cells viability became similar to the control probes, probably due to free radical protection.
Conclusion. Thus, FeFe2O4&Ag0 nanocomposite has apparent antiinflammatory properties and positive impact upon phagocytic component of immune system that enable its anticipated application for nosocomial infections treatment in patients of Resuscitation and Critical Care Units.