Damage of irradiation affects not only a definite person health, but his offspring in the form of genetic diseases as well. Approximately 56% of genetic diseases appear in the second generation. This research aim is an investigation functional activity of the small intestine from the 2-nd posterity of the irradiated male and intact female rats and comparison with the corresponding parameters of their 1st posterity.
Methods. The intact 2-months-old 2-nd male posterity of the irradiated by 0.5 Gr male rats was used. The accumulated fragments of small intestine mucosa from this posterity were incubated in the different substrates solutions due to the 1 hour in the oxygenated medium (t = 37 °С) according to O. M. Ugolev method. All substrates were prepared on the Ringer solution in the presence of bile. The hydrolytic and absorptive activity of the 2-nd posterity small intestine was detected.
Results. The level of the free glucose transport into the small intestine of the 2-nd posterity from the irradiated male rats was approximately the same with the same parameter for the intact group. It evidences not only high level of stability of glucose transport system and adaptive ability under changed environmental condition but adequacy of method and level of experiment. But the level of transport of M-glucose (product of maltose hydrolysis) was lower in F2 then in F1 in 25% and 3 times less unstable despite on the bigger amount of animals in the group (10 to 5 correspondingly). We suppose the different mechanisms for the transport of M-glucose and free glucose.
The level of free glycine transport in F2 group was by 42% less in comparison with F1 and by 22% less stable, but only by 6% less stable than in intact group on the same level of stability. The level of the transport of P-glycine (product of glycyl-glycine hydrolysys) in F2 was approximately the same with F1 and in the same way less than in intact group but in 2 times less stable than in both previous groups despite on the bigger amount of animals in the group (10 to 5 correspondingly). It means in the 2nd posterity of irradiated rats the destructive changes of the small intestine functional activity are accumulated first of all in the hydrolytic systems activity.
Conclusions. Irradiation of the rat’s males more than other injures hydrolytic systems in their posterity and results of irradiation of parents were detected in the 1-st posterity only for the protein nature substrates and in the 2-nd posterity — both for the protein and (first of all) carbohydrate nature substrates