Purpose: it is defined that gene polymorphisms influence on pharmacokinetics and pharmacodinamics of the majority of preparations. CYP3A4 is important for pharmacology, as it transforms about 50% of medical means. Thus, gene studying cyp3a4 represents considerable interest. Working out technology of definition of gene polymorphism experimentally occupies considerable volume of time and resources. Often enough works are conducted using the parametres obtained earlier that considerably narrows possibilities of researchers. With bioinformatic development the process specified above can be much more effective.
Methods: used nucleotide sequences of gene cyp3a4 Homo sapiens, found in gene bank NCBI by means of algorithm BLAST: nucleotide sequences levelled by means of Nidlman-Wunsh’s algorithm in the ClustalW program. Design of primers and in silico PCR, restriction analysis of amplicons and electrophores was conducted by means of Vector NTI 11 program.
Results: qualitative global alignment nucleotide sequences for the purpose of the gene analysis cyp3a4 was conducted, the marker technique is developed for the polymorphism and differentiation analysis allels this gene.
Conclusions: by means of bioinformation modelling programs polymorphism of the gene cyp3a4 in silico is investigated and parametres for the further studying allels gene are picked up. The presented results will allow to solve more effectively the problems put before pharmacogenetic.